Anti-SET7 antibody [s4E5]

• 产品名称

  Anti-SET7抗体[s4E5]
  参阅全部 SET7 一抗

• 描述

  小鼠单克隆抗体[s4E5] to SET7

• 宿主

  Mouse

• 特异性

  This antibody has been tested against recombinant SET7 but has not been tested against the endogenous protein yet.

• 经测试应用

  适用于: WB, ICC/IF, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Mouse, Human, Recombinant fragment
  

• 免疫原

  Recombinant full length protein corresponding to Human SET7.

• 阳性对照

• WB: Recombinant SET7, SET7/9 transfected 293T cell lysate. ICC/IF: HeLa cells. Flow Cyt (Intra): Jurkat cells; HeLa Cells.

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 10% Glycerol

• 纯度

  Tissue culture supernatant

• 纯化说明

  Purified from TCS.

• 克隆

  单克隆

• 克隆编号

  s4E5

• 骨髓瘤

  Sp2/0

• 同种型

  IgG2b

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab14820于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 80854 Human

• Entrez Gene: 73251 Mouse

• Omim: 606594 Human

• SwissProt: Q8WTS6 Human

• SwissProt: Q8VHL1 Mouse

• Unigene: 480792 Human

• Unigene: 192111 Mouse

• 别名

• H4 lysine 4 specific antibody

• Histone H3 K4 methyltransferase antibody

• Histone H3 lysine 4 specific methyltransferase antibody

• Histone H3-K4 methyltransferase SETD7 antibody

• Histone H4 K4 methyltransferase antibody

• Histone lysine N methyltransferase antibody

• Histone lysine N methyltransferase H3 lysine 4 specific SET7 antibody

• Histone-lysine N-methyltransferase SETD7 antibody

• KIAA1717 antibody

• KMT7 antibody

• Lysine methyltransferase antibody

• Lysine N-methyltransferase 7 antibody

• OTTHUMP00000164543 antibody

• OTTHUMP00000220049 antibody

• SET 7 antibody

• SET 7/9 antibody

• SET 9 antibody

• SET D7 antibody

• SET domain containing (lysine methyltransferase) 7 antibody

• SET domain containing protein 7 antibody

• SET domain containing protein 8 antibody

• SET domain-containing protein 7 antibody

• SET7 antibody

• SET7/9 antibody

• SET9 antibody

• Setd7 antibody

• SETD7_HUMAN antibody

• FLJ21193 antibody

• H3 K4 HMTase antibody

• H3-K4-HMTase SETD7 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SET7 antibody [s4E5] (ab14820)

  Immunocytochemistry/ Immunofluorescence analysis of SET7/9 in HeLa cells. The cell was stained with ab14820 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

• 

  Flow Cytometry (Intracellular) - Anti-SET7 antibody [s4E5] (ab14820)

  Flow cytometry analysis of SET7/9 in Jurkat cells. The cell was stained with ab14820 at 2-5µg for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

• 

  Western blot - Anti-SET7 antibody [s4E5] (ab14820)

  All lanes : Anti-SET7 antibody [s4E5] (ab14820) at 1/2000 dilution
  
  Lane 1 : 293T cell lysate
  Lane 2 : SET7/9 transfected 293T cell lysate
  
  Lysates/proteins at 5 µg per lane.
  
  Secondary
  All lanes : goat anti-mouse secondary antibody conjugated to HRP
  
  Predicted band size: 50 kDa
  
  

• 

  Western blot - Anti-SET7 antibody [s4E5] (ab14820)

  Anti-SET7 antibody [s4E5] (ab14820) at 1/1000 dilution + Recombinant human SET7/9 protein
  
  Secondary
  Goat anti-mouse secondary antibody conjugated to HRP
  
  Predicted band size: 50 kDa
  
  

• 

  Western blot - Anti-SET7 antibody [s4E5] (ab14820)

  Western blot analysis of recombinant SET7 using ab14820 at 1/1000. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system. Western blot analysis of recombinant SET7 using ab14820 at 1/1000. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system.

  This image was produced using the ascites version of this antibody. 

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SET7 antibody [s4E5] (ab14820)This image is courtesy of Katia Ancelin

  Immunoflouresence was carried out using mouse cells in culture. Conditions: 4% PFA fixation and dilution with 0.5% triton, 10mg/ml BSA in PBS. Dilution of ab14820 used: 1/50. Green = ab14820 detected with secondary antibody (Alexa 488). Blue = DAPI stain.

  This image was produced using the ascites version of this antibody. 

• 

  Flow Cytometry (Intracellular) - Anti-SET7 antibody [s4E5] (ab14820)

  Overlay histogram showing HeLa cells stained with ab14820 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14820, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  

  This image was produced using the ascites version of this antibody.