Anti-ATPB antibody [3D5] - Mitochondrial Marker

• 产品名称

  Anti-ATPB抗体[3D5] - Mitochondrial Marker
  参阅全部 ATPB 一抗

• 描述

  小鼠单克隆抗体[3D5] to ATPB - Mitochondrial Marker

• 宿主

  Mouse

• 特异性

  Human and Bovine complex V beta subunit (ATPB).

• 经测试应用

  适用于: WB, ICC/IF, Flow Cyt, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Human, Caenorhabditis elegans
  预测可用于: Goat, Cat, Dog, Pig, Common marmoset

• 免疫原

  Tissue, cells or virus corresponding to Human ATPB.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.4
  Preservative: 0.02% Sodium azide
  Constituent: HEPES buffered saline

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  IgG fraction

• 纯化说明

  Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

• 克隆

  单克隆

• 克隆编号

  3D5

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab14730于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• SwissProt: P00829 Cow

• SwissProt: P06576 Human

• SwissProt: P56480 Mouse

• SwissProt: P10719 Rat

• Unigene: 406510 Human

• Unigene: 238973 Mouse

• Unigene: 92965 Rat

• Entrez Gene: 327675 Cow

• Entrez Gene: 403669 Dog

• Entrez Gene: 506 Human

• Entrez Gene: 11947 Mouse

• Entrez Gene: 100157156 Pig

• Entrez Gene: 171374 Rat

• GenBank: BC016512 Human

• Omim: 102910 Human

• 别名

• ATP synthase subunit beta, mitochondrial antibody

• atp5b antibody

• ATPB antibody

• ATPB_HUMAN antibody

• ATPMB antibody

• ATPSB antibody

• Epididymis secretory protein Li 271 antibody

• HEL-S-271 antibody

• Mitochondrial ATP synthase beta subunit antibody

• Mitochondrial ATP Synthase Subunit Beta antibody

• Mitochondrial ATP synthetase beta subunit antibody

• ATP 5B antibody

• ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody

• ATP synthase subunit beta mitochondrial antibody

• 

  Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

  All lanes :
  
  Lane 1 : HeLa
  Lane 2 : HepG2
  Lane 3 : Hu liver
  Lane 4 : Ms liver
  
  Lysates/proteins at 20 µg per lane.
  
  Performed under reducing conditions.
  
  Predicted band size: 52 kDa
  
  
  
  

  Merged signal (red and green). Green - ab14730 observed at 52 kDa. Red - loading control, ab181602 observed at 37 kDa.

  Samples were subjected to SDS-PAGE. ab14730 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1ug/mL and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

  ab14730 staining ATPB in cultured human fibroblasts. Cells were fixed, permeabilized and then labelled with ab14730 followed by an AlexaFluor® 488-conjugated Goat anti-Mouse IgG1-specific secondary antibody (2 µg/ml)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

  ab14730 (2µg/ml) staining ATPB in human duodenum using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and mitochondrial staining of epithelium.
  Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  

• 

  Flow Cytometry - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

  ab14730 (blue) at 1µg/ml staining ATPB in HL-60 cells and analyzed by Flow cytometry. Red histogram represents equal quantity of isotype control.

• 

  Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

  All lanes : Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
  
  Lane 1 : isolated mitochondria from human heart at 5 µg
  Lane 2 : isolated mitochondria from bovine heart at 1 µg
  Lane 3 : isolated mitochondria from rat heart at 10 µg
  Lane 4 : isolated mitochondria from mouse heart at 10 µg
  
  Predicted band size: 52 kDa
  Observed band size: 52 kDa
  
  

• 

  Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

  All lanes : Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730) at 0.8 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 3 : Human liver tissue lysate - total protein (ab29889)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Predicted band size: 52 kDa
  Observed band size: 52 kDa