Anti-SDHB antibody [21A11AE7]

• 产品名称

  Anti-SDHB抗体[21A11AE7]
  参阅全部 SDHB 一抗

• 描述

  小鼠单克隆抗体[21A11AE7] to SDHB

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, IHC-Fr, WB, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Human
  预测可用于: Hamster, Pig, Zebrafish

• 免疫原

  Full length protein corresponding to Cow SDHB. Purified SDH from bovine heart.

• 阳性对照

• WB: Human, bovine, rat, and mouse heart mitochondria; Isolated mitochondria from HepG2 cells. IHC-Fr: Normal ageing human colon tissue. Flow Cyt: HEK-293 cells. ICC/IF: HeLa cells. HEK293 cells (HEK293-SDHB KO used as a negative cell line)

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  
  

  Product was previously marketed under the MitoSciences sub-brand.

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C.

• 存储溶液

  pH: 7.5
  Preservative: 0.02% Sodium azide
  Constituent: HEPES buffered saline

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  IgG fraction

• 纯化说明

  Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

• 克隆

  单克隆

• 克隆编号

  21A11AE7

• 同种型

  IgG2a

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab14714于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• SwissProt: P21912 Human

• SwissProt: Q9CQA3 Mouse

• SwissProt: Q007T0 Pig

• SwissProt: P21913 Rat

• SwissProt: A5PL98 Zebrafish

• Unigene: 465924 Human

• Unigene: 246965 Mouse

• Unigene: 3902 Rat

• Unigene: 75182 Zebrafish

• Entrez Gene: 286840 Cow

• Entrez Gene: 6390 Human

• Entrez Gene: 67680 Mouse

• Entrez Gene: 414412 Pig

• Entrez Gene: 298596 Rat

• Entrez Gene: 562149 Zebrafish

• Omim: 185470 Human

• SwissProt: Q3T189 Cow

• 别名

• Ip antibody

• Iron sulfur subunit antibody

• Iron sulfur subunit of complex II antibody

• Iron-sulfur subunit of complex II antibody

• mitochondrial antibody

• PGL 4 antibody

• PGL4 antibody

• SDH 1 antibody

• SDH antibody

• SDH1 antibody

• SDH2 antibody

• SDH2, homolog of antibody

• SdhB antibody

• SDHIP antibody

• Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial antibody

• Succinate dehydrogenase [ubiquinone] iron sulfur subunit antibody

• Succinate dehydrogenase [ubiquinone] iron-sulfur subunit antibody

• succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial antibody

• Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody

• Succinate Dehydrogenase 2, S. cerevisiae, homolog of antibody

• Succinate dehydrogenase complex iron sulfur subunit B antibody

• Succinate dehydrogenase complex subunit B iron sulfur antibody

• Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody

• succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody

• Succinate dehydrogenase iron sulfur protein antibody

• CWS2 antibody

• DHSB_HUMAN antibody

• FLJ92337 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SDHB antibody [21A11AE7] (ab14714)

  ab14714 staining SDHB in wild-type HEK293 cells (top panel) and SDHB knockout HEK293 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab14714 at 5µg concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Western blot - Anti-SDHB antibody [21A11AE7] (ab14714)

  All lanes : Anti-SDHB antibody [21A11AE7] (ab14714) at 5 µg/ml
  
  Lane 1 : Isolated mitochondria from Human heart at 5 µg
  Lane 2 : Isolated mitochondria from Bovine Heart at 1 µg
  Lane 3 : Isolated mitochondria from Rat heart at 10 µg
  Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
  Lane 5 : Isolated mitochondria from HepG2 (human liver hepatocellular carcinoma cell line) cells at 20 µg
  
  Secondary
  All lanes : Goat anti-Mouse secondary
  
  Observed band size: 28 kDawhy is the actual band size different from the predicted?
  Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
  
  
  
  Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

• 

  Immunohistochemistry (Frozen sections) - Anti-SDHB antibody [21A11AE7] (ab14714)

  ab14714 staining SDHB in normal ageing human colon tissue by Immunohistochemistry (Frozen sections).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SDHB antibody [21A11AE7] (ab14714)

  Mitochondrial localization of complex II visualized by immunocytochemistry using anti-complex II subunit 30 kDa Ip mAb 21A11 (ab14714). Cells were fixed, permeabilized and then labeled with ab14714 followed by an Alexa Fluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody.

• 

  Flow Cytometry - Anti-SDHB antibody [21A11AE7] (ab14714)

  Overlay histogram showing HEK-293 (human epithelial cell line from embryonic kidney) cells stained with ab14714 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14714, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SDHB antibody [21A11AE7] (ab14714)

  ICC/IF image of ab14714 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14714, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.