Anti-NDUFS3 antibody [17D95]

• 产品名称

  Anti-NDUFS3抗体[17D95]
  参阅全部 NDUFS3 一抗

• 描述

  小鼠单克隆抗体[17D95] to NDUFS3

• 宿主

  Mouse

• 经测试应用

  适用于: WB, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Human
  

• 免疫原

  Tissue, cells or virus. This information is considered to be commercially sensitive.

• 阳性对照

• Human heart mitochondria. In Flow Cytometry, this antibody gave a positive signal in methanol fixed/Tween permeabilised HepG2 cells.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  
  

  Product was previously marketed under the MitoSciences sub-brand.

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C.

• 存储溶液

  pH: 7.5
  Preservative: 0.02% Sodium azide
  Constituent: HEPES buffered saline

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  单克隆

• 克隆编号

  17D95

• 同种型

  IgG2a

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab14711于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 502528 Human

• Unigene: 30113 Mouse

• Entrez Gene: 287327 Cow

• Entrez Gene: 4722 Human

• Entrez Gene: 68349 Mouse

• Entrez Gene: 295923 Rat

• Omim: 603846 Human

• SwissProt: P23709 Cow

• SwissProt: O75489 Human

• SwissProt: Q9DCT2 Mouse

• 别名

• Complex I 30KD antibody

• Complex I 30kDa subunit antibody

• COMPLEX I, MITOCHONDRIAL RESPIRATORY CHAIN, 30-KD SUBUNIT antibody

• Complex I-30kD antibody

• mitochondrial antibody

• NADH coenzyme Q reductase antibody

• NADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa antibody

• NADH dehydrogenase [ubiquinone] iron sulfur protein 3 mitochondrial antibody

• NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 antibody

• NADH dehydrogenase ubiquinone 30 kDa subunit antibody

• NADH-ubiquinone oxidoreductase 30 kDa subunit antibody

• NADH-Ubiquinone Oxidoreductase Fe-S Protein 3 antibody

• NDUFS3 antibody

• NDUS3_HUMAN antibody

• CI 30 antibody

• CI 30KD antibody

• CI-30kD antibody

• 

  Western blot - Anti-NDUFS3 antibody [17D95] (ab14711)

  All lanes : Anti-NDUFS3 antibody [17D95] (ab14711) at 1 µg/ml
  
  Lane 1 : Human brain tissue lysate - total protein (ab29466)
  Lane 2 : Human heart tissue lysate - total protein (ab29431)
  Lane 3 : Heart (Mouse) Tissue Lysate
  Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 30 kDa
  Observed band size: 26 kDawhy is the actual band size different from the predicted?
  Additional bands at: 57 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 1 minute
  
  
  The band observed at 26 kDa could potentially be a cleaved form of NDUFS3 due to the presence of a 36 amino acid transit peptide.

• 

  Western blot - Anti-NDUFS3 antibody [17D95] (ab14711)Image is courtesy of an anonymous AbReview

  All lanes : Anti-NDUFS3 antibody [17D95] (ab14711) at 1/1000 dilution
  
  Lanes 1-2 : Whole cell lysates from NDUFS3 KO cells.
  Lanes 3-4 : Whole cell lysates from NDUFS3 WT cells.
  Lanes 5-6 : Isolated mitochondria from NDUFS3 KO cells.
  Lanes 7-8 : Isolated mitochondria from NDUFS3 WT cells.
  
  Lysates/proteins at 50 µg per lane.
  
  Secondary
  All lanes : Goat anti-mouse polyclonal HRP conjugate at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 30 kDa
  Additional bands at: 22 kDa (possible non-specific binding), 40 kDa (possible non-specific binding)
  
  
  Exposure time: 1 minute
  

  See Abreview

• 

  Flow Cytometry - Anti-NDUFS3 antibody [17D95] (ab14711)

  Overlay histogram showing HepG2 cells stained with ab14711 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14711, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Western blot - Anti-NDUFS3 antibody [17D95] (ab14711)

  All lanes : Anti-NDUFS3 antibody [17D95] (ab14711)
  
  Lane 1 : Isolated mitochondria from Human heart at 5 µg
  Lane 2 : Isolated mitochondria from Bovine heart at 4 µg
  Lane 3 : Isolated mitochondria from Rat heart at 10 µg
  Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
  
  Secondary
  All lanes : Goat anti-Mouse IgG
  
  Predicted band size: 30 kDa
  Observed band size: 26 kDawhy is the actual band size different from the predicted?
  
  
  
  Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.