Anti-S6K1抗体
参阅全部 S6K1 一抗
山羊多克隆抗体to S6K1
Goat
适用于: WB, IHC-Pmore details
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Cow, Dog, Xenopus laevis
Synthetic peptide: MISKRPEHLRMNL, corresponding to amino acids 490-502 of S6K1.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab14708于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 65-70 kDa (predicted molecular weight: 60 kDa). A 1 hour primary incubation is recommended for this product. | |
IHC-P | Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Unigene: 463642 Human
Unigene: 394280 Mouse
Unigene: 446624 Mouse
Unigene: 479484 Mouse
Unigene: 4042 Rat
Entrez Gene: 6198 Human
Entrez Gene: 72508 Mouse
Entrez Gene: 100009260 Rabbit
Omim: 608938 Human
SwissProt: P23443 Human
SwissProt: Q8BSK8 Mouse
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P70S6K1 antibody
p70S6Kb antibody
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Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody
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Ribosomal protein S6 kinase beta-1 antibody
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S6K-beta-1 antibody
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Serine/threonine kinase 14 alpha antibody
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STK14A antibody
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody (ab14708)
ab14708 (2µg/ml) staining of paraffin embedded Human Kidney shows staining in the cytoplasm-rich PCT while less so in the DCT and no signals in the glomerulus. following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.
Western blot - Anti-S6K1 antibody (ab14708)
Anti-S6K1 antibody (ab14708) at 1 µg/ml + NIH3T3 cell lysate (in RIPA buffer). at 35 µg
Predicted band size: 60 kDa
Detected by chemiluminescence.
Western blot - Anti-S6K1 antibody (ab14708)
Anti-S6K1 antibody (ab14708) at 0.3 µg/ml + Human Placenta lysate (in RIPA buffer) at 35 µg
Predicted band size: 60 kDa
Detected by chemiluminescence.
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