Anti-MTCO1 antibody [1D6E1A8]

• 产品名称

  Anti-MTCO1抗体[1D6E1A8]
  参阅全部 MTCO1 一抗

• 描述

  小鼠单克隆抗体[1D6E1A8] to MTCO1

• 宿主

  Mouse

• 经测试应用

  适用于: ICC, IHC-P, WB, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  预测可用于: Sheep, Goat, Cat, Dog, Pig, Caenorhabditis elegans, Zebrafish, Quail, Rhesus monkey, Chinese hamster, Common marmoset

• 免疫原

  Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• WB: HeLa, MCF7, C6, PC-12, Neuro-2a, RAW 264.7 whole cell lysates. Human skeletal, Rat heart and Rat liver tissue lysates. IHC-P: Human kidney, Mouse colon, and Rat colon tissue. ICC: Human cervical adenocarcinoma, Mouse neuroblastoma neuroblast, and rat glial tumor cells. Flow Cyt: HeLa, Neuro-2a, and C6 cells.

• 常规说明

  This product has switched from a hybridoma to recombinant production method on 24th May 2023.

  
  Western blot protocol advice:
  For best results with this antibody in Western blot, do not boil samples before loading onto the gel. Boiling of the sample will cause a loss of signal.

  Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.

  This antibody clone [1D6E1A8] is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at -20°C.

• 存储溶液

  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

• 浓度

• 100 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1.008 mg/ml

• 1 mg 浓度为 1.008 mg/ml

• 40 µg 浓度为 1.008 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  1D6E1A8

• 同种型

  IgG2a

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab14705于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• SwissProt: P00395 Human

• SwissProt: P00397 Mouse

• SwissProt: O79876 Pig

• SwissProt: P05503 Rat

• SwissProt: O78749 Sheep

• SwissProt: Q9MIY8 Zebrafish

• Entrez Gene: 2565700 Caenorhabditis elegans

• Entrez Gene: 4512 Human

• Entrez Gene: 17708 Mouse

• Entrez Gene: 26195 Rat

• Entrez Gene: 140539 Zebrafish

• Omim: 516030 Human

• SwissProt: P24893 Caenorhabditis elegans

• SwissProt: Q9ZZ64 Dog

• 别名

• COX1_HUMAN antibody

• COXI antibody

• Cytochrome c oxidase polypeptide I antibody

• Cytochrome c oxidase subunit 1 antibody

• Cytochrome C Oxidase subunit I antibody

• Mitochondrially encoded cytochrome c oxidase I antibody

• MT CO1 antibody

• MT-CO1 antibody

• MTCO 1 antibody

• MTCO1 antibody

• COI antibody

• COX I antibody

• COX1 antibody

• 

  Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

  All lanes : Anti-MTCO1 antibody [1D6E1A8] (ab14705) at 1/1000 dilution
  
  Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) non-mitochondrial fraction
  Lane 2 : HeLa mitochondrial fraction
  Lane 3 : MCF7(human breast adenocarcinoma epithelial cell) non-mitochondrial fraction
  Lane 4 : MCF7 mitochondrial fraction
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
  
  Observed band size: 35 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 5 seconds
  
  
  

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

• 

  Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

  All lanes : Anti-MTCO1 antibody [1D6E1A8] (ab14705) at 1/1000 dilution
  
  Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
  Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
  Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
  Lane 4 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
  Lane 5 : Human skeletal muscle tissue lysate
  Lane 6 : Rat heart tissue lysate
  Lane 7 : Rat liver tissue lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
  
  Observed band size: 35 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

  Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling MTCO1 with ab14705 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Human kidney. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval Solution2) for 20 mins. Counterstained with hematoxylin.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling MTCO1 with ab14705 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Mouse colon. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval Solution2) for 20 mins. Counterstained with hematoxylin.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

  Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling MTCO1 with ab14705 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Rat colon. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval Solution2) for 20 mins. Counterstained with hematoxylin.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human cervical adenocarcinoma epithelial cells labeling MTCO1 with ab14705 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Human cervical adenocarcinoma epithelial cells. ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse neuroblastoma neuroblast cells labeling MTCO1 with ab14705 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Mouse neuroblastoma neuroblast cells. ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat glial tumor cells labeling MTCO1 with ab14705 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Rat glial tumor cells. ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

• 

  Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

  Flow cytometric analysis of Human HeLa cells labelling MTCO1 with ab14705 at 1/500 dilution (Right) compared with an isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at was used as the secondary antibody. Gated on viable cells.

• 

  Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

  Flow cytometric analysis of Neuro-2a cells labelling MTCO1 with ab14705 at 1/500 dilution (Right) compared with an isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at was used as the secondary antibody. Gated on viable cells.

• 

  Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

  Flow cytometric analysis of C6 cells labelling MTCO1 with ab14705 at 1/500 dilution (Right) compared with an isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at was used as the secondary antibody. Gated on viable cells.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)Image from Phillips J et al., Sci Rep. 2016 May 16;6:26013. Fig 3b doi: 10.1038/srep26013. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

  This image was generated using a previous batch manufactured using hybridoma production method.

  250 μm human cerebellar sections from control individuals and a patient with mitochondrial disease underwent passive clearing at 37 °C for 2 or 4 weeks.

  The quality of immunofluorescent staining is determined by duration of passive clearing; 2 weeks of passive clearing produced minimal labelling of the white matter in the granule cell layer (NF-H; green; 488 nm and MBP; red, 546 nm) with an absence of labelling of mitochondria (MTCO1 (COXI) (ab14705, 1/100); purple; 647 nm; Extending passive clearing to 4 weeks improved the quality of stain with identifiable Purkinje cells and their axons (NF-H, green; 488 nm) and their myelin sheaths (MBP; red, 546 nm) and mitochondria (MTCO1 (COXI) (ab14705, 1/100); purple; 647 nm.