Anti-SGLT1抗体
参阅全部 SGLT1 一抗
兔多克隆抗体to SGLT1
Rabbit
适用于: WB, ELISA, Dot blot, IPmore details
不适用于: IHC-P
与反应: Rat, Human
Synthetic peptide corresponding to Human SGLT1 aa 402-422. Synthetic peptide, corresponding to amino acids 402-422 of Human SGLT1
Database link: P13866
(Peptide available as ab190911)
WB: Rat enterocytes, rat heart, and human skeletal muscle tissues lysates.
A blocking peptide for this product is available as ab190911.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituents: Tris glycine, 0.5% BSA, HEPES, 30% Glycerol
Immunogen affinity purified
Purified on antigen sepharose affinity column
多克隆
IgG
Abpromise™承诺保证使用ab14686于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (6) | 1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa). |
ELISA | Use at an assay dependent concentration. | |
Dot blot | Use at an assay dependent concentration. | |
IP | Use at an assay dependent concentration. |
应用说明
Is unsuitable for IHC-P.
Entrez Gene: 6523 Human
Omim: 182380 Human
SwissProt: P13866 Human
Unigene: 1964 Human
Unigene: 10224 Rat
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Western blot - Anti-SGLT1 antibody (ab14686)
All lanes : Anti-SGLT1 antibody (ab14686) at 1/500 dilution
Lane 1 : Rat enterocytes
Lane 2 : Rat heart tissue
Lane 3 : Human skeletal muscle tissue
Predicted band size: 73 kDa
Tissue extracts were made in Solobuffer with protease inhibitors. The samples were solubilized in SDS-PAGE sample buffer and reduced with 2.5% BME.
Total 40-50ug protein was applied per lane on 10% SDS PAGE gels.
The proteins were transferred to nitrocellulose F and blocked with 5% milk and blotted with 1:500 diluted ab14686.
The image was obtained with high sensitivity ECL assay and Image was captured with a CCD cooled camera for 30 sec.
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