Anti-SGLT1抗体
参阅全部 SGLT1 一抗
兔多克隆抗体to SGLT1
Rabbit
The immunogen used for this product shares 75% homology with SGLT5. Cross-reactivity with this protein has not been confirmed experimentally.
适用于: IHC-Pmore details
与反应: Human
Synthetic peptide corresponding to Human SGLT1 aa 600-700.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituents: Tris glycine, 0.5% BSA, HEPES, 30% Glycerol
浓度
50 µg 浓度为 0.75 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab14685于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | Use a concentration of 2 µg/ml. |
Entrez Gene: 6523 Human
Omim: 182380 Human
SwissProt: P13866 Human
Unigene: 1964 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody (ab14685)
ab14685 (2µg/ml) staining SGLT1 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the apical epithelium cells of the villi.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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