Anti-PDE5A/PDE5抗体
参阅全部 PDE5A/PDE5 一抗
兔多克隆抗体to PDE5A/PDE5
Rabbit
This antibody detects 99 and 89 kDa PDE5A/PDE5 proteins in various tissues.
适用于: IHC-P, IP, WB, ICC/IFmore details
与反应: Mouse, Rat, Human
Synthetic peptide corresponding to Human PDE5A/PDE5. Synthetic peptide common to all PDE5A variants, taken within amino acid region 120-170 of human PDE5A protein.
(Peptide available as ab150691)
Storage of very dilute antibody solutions is not recommended.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
浓度
批次浓度范围 50 µg 浓度为 0.68 - 0.72 mg/ml
Immunogen affinity purified
Purified on antigen sepharose affinity column.
多克隆
IgG
Abpromise™承诺保证使用ab14672于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | 1/10. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
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Unigene: 134911 Mouse
Unigene: 10861 Rat
Unigene: 133138 Rat
Entrez Gene: 8654 Human
Entrez Gene: 242202 Mouse
Omim: 603310 Human
SwissProt: O76074 Human
SwissProt: Q8CG03 Mouse
Unigene: 647971 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody (ab14672)
ab14672 (4µg/ml) staining PDE5A/PDE5 in human oesophagus using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasm and nuclei in stratified squamous epithelium cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunocytochemistry/ Immunofluorescence - Anti-PDE5A/PDE5 antibody (ab14672)
ICC/IF image of ab14672 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14672, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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