HeLa whole cell lysate (TNF alpha stimulated)

产品名称

HeLa全细胞裂解物(TNF alpha stimulated)
参阅全部 HeLa 细胞裂解液

常规说明

The HeLa whole cell extracts were collected in Lysis Buffer after a 30 minute incubation with or without TNF alpha (20 ng/ml). The protein content was determined by a Bradford based assay. We recommend aliquoting the extracts into single use fractions and then storing them.

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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Mycoplasma free

Yes

形式

Liquid

存放说明

Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

存储溶液

pH: 7.50
Constituents: 0.004% EGTA, 0.01% Magnesium chloride, 0.48% HEPES, 0.015% EDTA, 20% Glycerol, 2.05% Sodium chloride, 1% Igepal

浓度

Functional Studies - HeLa whole cell lysate (TNF alpha stimulated) (ab14658)

Ab14658 has been tested for NFkappaB activation. The signal intensity for NFkappaB activation is compared to the signal intensity obtained with extracts from unstimulated HeLa cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of NFkappaB activation in a given cell type.