HeLa whole cell lysate (IL1 alpha stimulated)

产品名称

HeLa全细胞裂解物(IL1 alpha stimulated)
参阅全部 HeLa 细胞裂解液

常规说明

The HeLa whole cell extracts were collected in Lysis Buffer after a 30 minute incubation with or without IL1 alpha (5 ng/ml). The protein content was determined by a Bradford based assay. We recommend aliquoting the extracts into single use fractions and then storing them.

Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Mycoplasma free

Yes

形式

Liquid

存放说明

Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

存储溶液

pH: 7.50
Constituents: 0.004% EGTA, 0.01% Magnesium chloride, 0.48% HEPES, 0.015% EDTA, 20% Glycerol, 2.05% Sodium chloride, 1% Igepal

浓度

HeLa whole cell lysate (IL1 alpha stimulated) (ab14656)

Ab14656 tested for NFkappaB activation. The signal intensity for NFkappaB activation is compared to the signal intensity obtained with extracts from unstimulated HeLa cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of NFkappaB activation in a given cell type.