HeLa nuclear extract lysate (TPA stimulated 2 hr)

• 产品名称

  HeLa核extract裂解物(TPA stimulated 2 hr)
  参阅全部 HeLa 细胞裂解液

• 常规说明

  Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12 myristate, 13 acetate) for two hours at 37°C immediately prior to harvesting. We recommend aliquoting the extracts into single use fractions and then storing.

• 经测试应用

  适用于: WB, EMSAmore details

• Mycoplasma free

  Yes

• 形式

  Liquid

• 存放说明

  Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.90
  Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol

• 浓度

• 200 µg 浓度为 5 mg/ml

The Abpromise guarantee

Abpromise™承诺保证使用ab14653于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。