HeLa nuclear extract lysate (TNF alpha stimulated)

产品名称

HeLa核extract裂解物(TNF alpha stimulated)
参阅全部 HeLa 细胞裂解液

常规说明

The HeLa nuclear extracts have been collected in Lysis Buffer after a 30 minute incubation with or without TNF alpha. The protein content has been determined by a Bradford based assay (20 ng/ml). We recommend aliquoting the extracts into single use fractions and then storing.

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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Mycoplasma free

Yes

形式

Liquid

存放说明

Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

存储溶液

pH: 7.5
Constituents: 0.004% EGTA, 0.01% Magnesium chloride, 0.077% DTT, 0.48% HEPES, 0.015% EDTA, 20% Glycerol, 2.05% Sodium chloride, 1% Ethoxy Ethanol

With Protease inhibitors

浓度

Functional Studies - HeLa nuclear extract lysate (TNF alpha stimulated) (ab14652)

Each lot has been tested for NFkappaB activation. The signal intensity for NFkappaB activation in each lot is compared to the signal intensity obtained with extracts from unstimulated HeLa cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of NFkappaB activation in a given cell type.