Anti-PDE4抗体
参阅全部 PDE4 一抗
兔多克隆抗体to PDE4
Rabbit
This antibody detects all known PDE4 A and D variants including PDE4A1, A5, Ax, A8 and PDE4D1-D5. It has low affinity for PDE4B variants.
适用于: WB, ICC/IFmore details
与反应: Mouse, Rat, Human
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Rat brain.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Thimerosal (merthiolate)
浓度
批次浓度范围 50 µg 浓度为 0.7 - 0.78 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab14628于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent dilution. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Entrez Gene: 5141 Human
Entrez Gene: 5142 Human
Entrez Gene: 5143 Human
Entrez Gene: 5144 Human
SwissProt: P27815 Human
SwissProt: Q07343 Human
SwissProt: Q08493 Human
SwissProt: Q08499 Human
PDE4D antibody
Phosphodiesterase 4 antibody
Phosphodiesterase 4A antibody
Phosphodiesterase 4B antibody
Phosphodiesterase 4C antibody
Phosphodiesterase 4D antibody
PDE4A antibody
PDE4B antibody
PDE4C antibody
Western blot - Anti-PDE4 antibody (ab14628)
ab 14628 detecting 3 isoforms of PDE4A subclasses (PDE4A1, PDE4A5 and PDE4Ax), two isoforms of PDE4D (PDE4D1, PDE4D3) in rat brain by Western blot.
ab 14628 detecting 3 isoforms of PDE4A subclasses (PDE4A1, PDE4A5 and PDE4Ax) and two isoforms of PDE4D (PDE4D1, PDE4D3) in rat brain by Western blot.
Immunocytochemistry/ Immunofluorescence - Anti-PDE4 antibody (ab14628)
ICC/IF image of ab14628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14628, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
