Anti-Desmoglein 3/PVA antibody [5G11]

• 产品名称

  Anti-Desmoglein 3/PVA抗体[5G11]
  参阅全部 Desmoglein 3/PVA 一抗

• 描述

  小鼠单克隆抗体[5G11] to Desmoglein 3/PVA

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• IHC-P: FFPE human skin melanoma tissue sections.

• 常规说明

  The clone number has been updated from 3G133 to 5G11 since both name the same clone and 5G11 is the original clone number.

  For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 10 µg 浓度为 1 mg/ml

• 批次浓度范围 100 µg 浓度为 0.8 - 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  5G11

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab14416于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1830 Human

• Omim: 169615 Human

• SwissProt: P32926 Human

• Unigene: 1925 Human

• 别名

• Cadherin family member 6 antibody

• CDHF 6 antibody

• CDHF6 antibody

• Desmoglein 3 antibody

• Desmoglein 3 precursor antibody

• Desmoglein-3 antibody

• Desmoglein3 antibody

• DKFZp686P23184 antibody

• DSG 3 antibody

• DSG3 antibody

• DSG3_HUMAN antibody

• Pemphigus vulgaris antigen antibody

• PVA antibody

• 130 kD pemphigus vulgaris antigen antibody

• 130 kDa pemphigus vulgaris antigen antibody

• Balding antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (ab14416)

  IHC image of Desmoglein 3/PVA staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14416, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

   

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (ab14416)This image is courtesy of an anonymous Abreview

  Immunohistochemical analysis of Human tongue tissue, staining Desmoglein 3/PVA with ab14416.
  
  Tissue was fixed with formaldehyde and antigen retrieval was by proteinase K. Samples were incubated with primary antibody (1/500 in Tris buffered saline with 0.1% Tween 20) for 18 hours at 4°C. An HRP-conjugated goat anti-mouse polyclonal IgG (1/2) was used as the secondary antibody.

  See Abreview