Anti-Smt3抗体
兔多克隆抗体to Smt3
Rabbit
适用于: WB, ELISAmore details
与反应: Saccharomyces cerevisiae
Recombinant protein (S. cerevisiae).
Most yeast cell lysates can be used as a positive control without induction or stimulation.
Centrifuge product if not completely clear after standing at room temperature.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
250 µg 浓度为 1 mg/ml
Ion Exchange Chromatography
Anti-Sumo Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
多克隆
IgG
Abpromise™承诺保证使用ab14405于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | 1/1000. Detects a band of approximately 12 kDa. Note: Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. |
| ELISA | (1) | 1/1000 - 1/5000. |
Entrez Gene: 852122 Saccharomyces cerevisiae
SwissProt: Q12306 Saccharomyces cerevisiae
SMT3_YEAST antibody
Ubiquitin like protein of the SUMO family antibody
Ubiquitin like protein SMT3 antibody
Ubiquitin-like protein SMT3 antibody
DmSUMO 1 antibody
Small Ubiquitin-like modifier antibody
SMT3 antibody
Western blot - Anti-Smt3 antibody (ab14405)
All lanes : Anti-Smt3 antibody (ab14405) at 1/1000 dilution
Lanes 1 & 3 & 5 & 7 & 9 & 11 & 13 : Lysate prepared from E.coli expressed Yeast SUMO protein and SUMO-GFP fusion protein after incubation with lysed insect cells
Lanes 2 & 4 & 6 & 8 & 10 & 12 & 14 : Lysate prepared from SUMO-GFP fusion protein after incubation with lysed insect cells in the presence of 2% SDS
Lysates/proteins at 2 µg per lane.
Western blot of SUMO-GFP fusion proteins cleaved by insect cell protein extracts. Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins.
ELISA - Anti-Smt3 antibody (ab14405)This image is courtesy of an anonymous Abreview
ab14405 detecting Smt3 in Escherichia coli recombinant protein (BL21(DE3) by Direct ELISA. Wells were coated with 10µg each of diluted control protein (without SUMO fusion - Negative Controls) and target protein (with SUMO fusion protein) in bicarbonate buffer. Wells were blocked with 5% milk for 1 hour at room temperature. The samples were incubated with primary antibody (1/5000 in TBST +5% milk) for 45 minutes. An HRP-conjugated goat polyclonal to rabbit IgG (1/40000) was used as the secondary antibody.
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