Anti-MSH6 antibody [44]

• 产品名称

  Anti-MSH6抗体[44]
  参阅全部 MSH6 一抗

• 描述

  小鼠单克隆抗体[44] to MSH6

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, WB, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide, corresponding to amino acids 225-333 of Human MSH6

• 阳性对照

• WB: HeLa, A431 and HAP1 (HAP1-MSH6 knockout cell lysate used as negative control) cell lysates. IHC-P: Human colon carcinoma tissue. ICC/IF: HeLa cells and HAP1 cells (HAP1-MSH6 knockout cells used as negative cell line).

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.3
  Preservative: 0.05% Sodium azide
  Constituent: 1% BSA

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  44

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab14204于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 2956 Human

• Omim: 600678 Human

• SwissProt: P52701 Human

• Unigene: 445052 Human

• 别名

• GTBP antibody

• GTMBP antibody

• hMSH6 antibody

• HNPCC 5 antibody

• HNPCC5 antibody

• HSAP antibody

• MSH 6 antibody

• MSH6 antibody

• MSH6_HUMAN antibody

• mutS (E. coli) homolog 6 antibody

• MutS alpha 160 kDa subunit antibody

• MutS homolog 6 (E. coli) antibody

• mutS homolog 6 antibody

• MutS-alpha 160 kDa subunit antibody

• p160 antibody

• Sperm associated protein antibody

• DNA mismatch repair protein Msh6 antibody

• G/T mismatch binding protein antibody

• G/T mismatch-binding protein antibody

• 

  Western blot - Anti-MSH6 antibody [44] (ab14204)

  All lanes : Anti-MSH6 antibody [44] (ab14204) at 1/500 dilution
  
  Lane 1 : Wild-type HeLa cell lysate
  Lane 2 : MSH6 knockout HeLa cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Performed under reducing conditions.
  
  Observed band size: 160 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Lanes 1- 2: Merged signal (red and green). Green - ab14204 observed at 160 kDa. Red - Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) observed at 50 kDa.

   ab14204 was shown to react with MSH6 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255410 (knockout cell lysate ab263763) was used. Wild-type HeLa and MSH6 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab14204 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody [44] (ab14204)

  ab14204 staining MSH6 in wild-type HAP1 cells (top panel) and MSH6 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab14204 at 1/250 dilution and ab202272 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Western blot - Anti-MSH6 antibody [44] (ab14204)

  All lanes : Anti-AMACR + p63 antibody [4A4 (p63)] (ab14202)
  
  Lane 1 : Wild-type HAP1 cell lysate
  Lane 2 : MSH6 knockout HAP1 cell lysate
  Lane 3 : HeLa cell lysate
  Lane 4 : A431 cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  
  

  Lanes 1 - 4: Merged signal (red and green). Green - ab14204 observed at 160 kDa. Red - loading control, ab18251, observed at 52 kDa.

  
  ab14204 was shown to specifically react with MSH6 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when MSH6 knockout HAP1 samples were used. Wild-type and MSH6 knockout samples were subjected to SDS-PAGE. ab14204 and ab18251 (loading control to alpha Tubulin) were diluted at 1/100 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody [44] (ab14204)

  ab14204 staining MSH6 in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab14204 at 1/250dilution and ab202272 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [44] (ab14204)

  ab14204 staining human colon carcinoma by IHC-P.