Propidium Iodide

• 产品名称

  Propidium Iodide

• 产品概述

  Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized.

  
  

  For Microscopy analysis: PI can be viewed using rhodamine(red) filter (λ= 536/617). Cells will only be stained if the membrane has been permeated, either naturally (non-viable cells) or with detergents (for fluorescent staining).

  
  

  For Flow Cytometry analysis: PI staining can be monitored in FL2 channel.

  
  

  If used together as control for Annexin V assays ab14082, ab14083 or ab14152, PI should be diluted to 250 µg/ml solution (in PBS) prior use and added as 1 µl/ Annexin V Assay (0.25µg/assay).

  
  

  Visit our FAQs page for tips and troubleshooting.

• 说明

  This product is manufactured by BioVision, an Abcam company and was previously called 1056 Propidium Iodide. 1056-1 is the same size as the 1 ml size of ab14083.

• 
  

• 存放说明

  Store at +4°C. Please refer to protocols.

• 组件	标识符	1 ml
  Propidium Iodide (1mg/ml)	ab14083	1 x 1ml

The Abpromise guarantee

Abpromise™承诺保证使用ab14083于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 

  Flow cytometry: ab14083Image from Wang et al., PLoS One., 10(9). Fig 3a & c. doi:10.1371/journal.pone.0137712 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Wang et al. used ab14083 to investigate an optimized platform for maintaining proliferation of giant panda mesenchymal stem cells (MSCs). MSCs were fixed in 70% alcohol and 30% PBS at 4°C for 1 hour. Cells are then incubated in the dark with PBS, 20µg/ml propidium iodide (ab14083) and 1% RNaseA for 30 minutes at 37°C. Cell samples are resuspended in PBS and analyzed using FACS Calibur flow cytometry.

  Flow cytometry analysis shows the cells are at different cell cycle stages in different concentrations (0ng/ml and 5ng/ml respectively) of basic fibroblast growth factor (bFGF).

   

• 

  Immunofluorescence analysis using ab14083Image from Melia MM et al ., Plos One.,29 (8). Fig 1b DOI: 10.1371/journal.pone.0106281. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Vero-DogSLAM (VDS) cells and Vero (African Green Monkey kidney) cells were infected with wtCDV. Cells were fixed, permeabilised and stained with SSPE serum and rabbit anti-human FITC; nuclei were stained using ab14083 propidium iodide. Images were taken using a Nikon Eclipse TE2000-U UV microscope (x400).