Anti-FABP4抗体
参阅全部 FABP4 一抗
兔多克隆抗体to FABP4
Rabbit
适用于: IHC-Pmore details
与反应: Human
Recombinant full length protein (Human).
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.134% PBS, 0.58% Sodium chloride
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab13979于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | 1/200. |
Entrez Gene: 2167 Human
Omim: 600434 Human
SwissProt: P15090 Human
Unigene: 391561 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)
Immunohistochemical analysis of FFPE human adipose tissue, labeling FABP4 with ab13979 (2µg/ml). DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)Image from Holm S et al., PLoS One. 2011;6(12):e28785. Epub 2011 Dec 9. Fig 3.; doi:10.1371/journal.pone.0028785; December 9, 2011, PLoS ONE 6(12): e28785.
Immunohistochemical analysis of Human carotid atherosclerotic plaques, staining FABP4 with ab13979.
Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6) and blocked with 0.5% BSA. Sections were incubated with primary antibody for 1 hour at room temperature. Staining was detected using DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)This image is courtesy of an anonymous Abreview.
Immunohistochemical analysis of FFPE mouse pancreatic and peri-pancreatic fatty tissue, labeling FABP4 with ab13979. Tissue was fixed in 10% neutral buffered formalin, heat mediated antigen retrieval was performed using citrate buffer, and blocking was performed with 10% goat serum and 1% BSA inj TBST for 1 hour at 25°C.
Staining with ab13979 (diluted 1/500) was performed for 12 hours at 4°C. A goat anti-rabbit HRP-conjugated secondary was used, diluted 1/200.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)Image from Holm S et al., PLoS One. 2011;6(12):e28785. Epub 2011 Dec 9. Fig 3.; doi:10.1371/journal.pone.0028785; December 9, 2011, PLoS ONE 6(12): e28785.
Immunohistochemical analysis of Human carotid atherosclerotic plaques, staining FABP4 with ab13979.
Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6) and blocked with 0.5% BSA. Sections were incubated with primary antibody for 1 hour at room temperature. An AlexaFluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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