Anti-GFP抗体
参阅全部 GFP 一抗
鸡多克隆抗体to GFP
Chicken
Our GFP antibody does cross-react with the many fluorescent proteins that are derived from the jellyfish Aequorea victoria. These are all proteins that differ from the original GFP by just a few point mutations (EGFP, YFP, mVenus, CFP, BFP etc.).
适用于: WB, ICC/IFmore details
与反应: Species independent
Recombinant full length protein corresponding to GFP.
Database link: P42212
ICC: GFP-transfected NIH/3T3 (Mouse embryo fibroblast cell line). WB: Transgenic mouse spinal cords.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol, 0.16% Sodium phosphate
浓度
100 µl 浓度为 10 mg/ml
IgY fraction
Sterile filtered.
多克隆
IgY
Abpromise™承诺保证使用ab13970于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (13) | 1/5000. |
| ICC/IF | (22) | 1/2000. Used at a dilution of 1/2000 for 1 hr (see Abreview for further information). |
GFP antibody
Green fluorescent protein antibody
Western blot - Anti-GFP antibody (ab13970)This image is courtesy of an anonymous Abreview
All lanes : Anti-GFP antibody (ab13970) at 1/2000 dilution (Diluent 1x TBS /4 hours at 4°C)
Lane 1 : 3 µg of GFP plasmid overexpressed in mouse cardiomyocytes whole cell lysate with BSA / for 1 hour at room temperature
Lane 2 : 2 µg of GFP plasmid overexpressed in mouse cardiomyocytes whole cell lysate with BSA / for 1 hour at room temperature
Lane 3 : 1 µg of GFP plasmid overexpressed in mouse cardiomyocytes whole cell lysate with BSA / for 1 hour at room temperature
Lysates/proteins at 25 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176) at 1/5000 dilution
Performed under reducing conditions.
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Gel Running Conditions: Reduced Denaturing (15% PAGE)
Detection method: Fluorescent Secondary Antibodies
Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970)This image is courtesy of an Abreview submitted by Christophe Lachaud
ab13970 staining GFP in U-2 OS (Human bone osteosarcoma epithelial cell line) cells by ICC/IF.
Cells were paraformaldehyde fixed, permeabilized with 0.5% triton and blocked with 2% antibody dilution buffer for 2 hours. Cells were incubated with the primary antibody (1/1000) for 1 hour at 25°C. An undiluted Alexa Fluor® 488 conjugated Goat anti-chicken polyclonal was used as the secondary antibody.
Western blot - Anti-GFP antibody (ab13970)Image courtesy of an anonymous Abreview.
All lanes : Anti-GFP antibody (ab13970) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
Lane 2 : Whole cell lysate prepared from HeLa cells.
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : IRDye 800CW conjugated goat anti-chicken polyclonal at 1/15000 dilution
Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970)
Transgenic mice expressing GFP selectively in lamina II of the spinal cord.
In the right panels, note the correspondance between the green (rabbit anti-GFP) and red signals (chicken anti-GFP from Abcam) indicating that these two antibody preparations recognized the same gene product. The secondary antibody used with ab13970 was an FITC-labeled goat anti-chicken
Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970)
ab13970 staining GFP in GFP-transfected NIH/3T3 (Mouse embryo fibroblast cell line) cells.
The cells were fixed with 4% formaldehyde (10 minutes) and then blocked in 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1 hour. The cells were then incubated with ab13970 at 1/2000 dilution overnight at +4°C followed by incubation with Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (ab150173), for 1 hour, at 1 μg/ml.
Under identical experimental conditions, when compared to the basal level of GFP expression in transfected NIH/3T3 cells, the cells upon which ab13970 was applied gave a stronger signal in the 488 channel, indicating that ab13970 is binding to GFP and therefore eliciting signal amplification.
ab13970 was also applied to non-GFP-transfected NIH/3T3 cells, which produced no positive staining, indicating specificity for GFP.
Nuclear DNA was labeled with 1.43 μM DAPI (blue).
Western blot - Anti-GFP antibody (ab13970)
Lane 1 : Rabbit anti-GFP
Lane 2 : Anti-GFP antibody (ab13970)
All lanes : Transgenic mouse spinal cords
Western blot of transgenic mouse spinal cords showing that the rabbit anti-GFP (lane 1) and the chicken anti-GFP (Abcam; lane 2) recognize a band at the same molecular weight.
Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970)This image is courtesy of an Abreview submitted by Dr Radbod Darabi
ab13970 staining GFP + tumor in mouse muscle cells by ICC/IF.
Cells were formaldehyde fixed and blocked with 3% BSA for 1 hour at 24°C prior to incubation with the primary antibody (1/500) for 1 hour at 24°C. An Alexa Fluor® 488 conjugated goat anti-chicken was used as the secondary.
Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970)Image courtesy of an AbReview submitted by Dr Francois Daubeuf.
Immunocytochemical/ Immunoflurescence analysis of cytospined HEK-293 cells (Human epithelial cell line from embryonic kidney) transfected with GFP, labeling GFP with ab13970 at 1/200 incubated for 16 hours at 4°C with 1% BSA in PBS. Secondary used was a donkey anti-chicken polyclonal DyLight® 594 at 1/500.
GFP is shown in red (DyLight® 594).
Nuclei are counterstained in blue (DAPI).
The left panel shows HEK-293 cells transfected with GFP and the right panel shows non-transfected HEK-293 cells.
