Anti-TLR3 antibody [40C1285]

• 产品名称

  Anti-TLR3抗体[40C1285]
  参阅全部 TLR3 一抗

• 描述

  小鼠单克隆抗体[40C1285] to TLR3

• 宿主

  Mouse

• 特异性

  Negative results in WB have been obtained for mouse interstine lysates (human intestine is a positive control). The protein is heavily glycosylated and the observed band size should be higher than the predicted 103kDa. The protein is found at different molecular weight in different tissues so optimization may be necessary.

• 经测试应用

  适用于: IHC-P, Flow Cyt (Intra), WBmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Synthetic peptide corresponding to Human TLR3 aa 50-150 conjugated to keyhole limpet haemocyanin.
  Database link: O15455

  Run BLAST with Run BLAST with 

• 阳性对照

• Human intestine tissue lysate, mouse spleen Flow Cyt (Intra): human B cells, human monocytes, SW480, SW620 (colon cancer)

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.4
  Preservative: 0.05% Sodium azide
  Constituents: PBS, 0.05% BSA

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  40C1285

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab13915于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7098 Human

• Entrez Gene: 142980 Mouse

• Omim: 603029 Human

• SwissProt: O15455 Human

• SwissProt: Q99MB1 Mouse

• Unigene: 657724 Human

• Unigene: 33874 Mouse

• 别名

• TLR 3 antibody

• Tlr3 antibody

• TLR3_HUMAN antibody

• Toll Like Receptor 3 antibody

• Toll-like receptor 3 antibody

• CD283 antibody

• CD283 antigen antibody

• IIAE2 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [40C1285] (ab13915)

  Immunohistochemical analysis of formalin fixed paraffin embedded tissue section of mouse intestine using ab13915 at 1/500 dilution with HRP-DAB detection and hematoxylin counterstaining. The image shows a punctate staining of the ER and endosomes in a subset of cells in Peyer's patches (organized lymphoid nodules) in the tested section.

• 

  Western blot - Anti-TLR3 antibody [40C1285] (ab13915)

  All lanes : Anti-TLR3 antibody [40C1285] (ab13915) at 3 µg/ml
  
  Lane 1 : Untransfected HEK293 cell lysate
  Lane 2 : HEK293 cell lysate transfected with human TLR3 cDNA whole cell lysate
  Lane 3 : Human intestine tissue lysate
  Lane 4 : Human placenta tissue lysate
  Lane 5 : Human heart tissue lysate
  Lane 6 : Human ovary tissue lysate
  
  Secondary
  All lanes : Goat anti-mouse HRP conjugate
  
  Predicted band size: 108 kDa
  
  

• 

  Flow Cytometry (Intracellular) - Anti-TLR3 antibody [40C1285] (ab13915)

  Flow cytometry (Intracellular) analysis of human B cells using ab13915 at 1µg/106 cells followed by FITC conjugated secondary antibody (Red). Green shows isotype control.  

• 

  Western blot - Anti-TLR3 antibody [40C1285] (ab13915)

  Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20 µg/lane human intestine tissue lysate (lane C).

  Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20 µg/lane human intestine tissue lysate (lane C).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [40C1285] (ab13915)

  Immunohistochemical analysis of formalin fixed paraffin embedded tissue section of mouse colon using ab13915 at 1/500 dilution with HRP-DAB detection and hematoxylin counterstaining. Intense signal was found in subset of cells at the bases of the crypts.

• 

  Flow Cytometry (Intracellular) - Anti-TLR3 antibody [40C1285] (ab13915)

  ab13915 at 2µg / 1x106 cells staining intracellular TLR3 in human monocytes by Flow Cyt (Intra). A PE-conjugated goat polyclonal to mouse IgG1 was used as secondary. The shaded histogram repersents monocytes without antibody; green repersents isotype control and red histogram shows staining with ab13915. 

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [40C1285] (ab13915)

  ab13915 at 5ug/ml staining TLR3 in mouse spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). The left image repersents staining with isotype control and right one repersents staining with ab13915.
  

• 

  Flow Cytometry (Intracellular) - Anti-TLR3 antibody [40C1285] (ab13915)This image is a courtesy of Tanja Matijevic

  ab13915 staining TLR3 in SW480, SW620 (colon cancer) and FaDu, Detroit 562 obtained from human cells by Flow Cytometry. Samples were prepared by trypsinizining and washing in PBS and centrifugation in a buffer containing 2% FBS, 0.1% Na-azide and PBS. Cells were fixed in paraformaldehyde and permeabilized in 0.2% Tween. Gating and analysis was performed by WinMDI 2.9 software. The primary antibody was diluted to 1 µg/cells (PBS, 2% FBS and 0.1% Na-azide) and incubated with sample for 30 minutes at 4°C. An FITC conjugated rabbit polyclonal to mouse IgG, diluted to 1/40, was used as secondary. Image represents FACS analysis of TLR3 protein expression in SW480 (A), SW620 (B), FaDu (C) and Detroit 562 (D) cell lines. Cell lines were permeabilized and immunostained with TLR3-specific antibody (thick black l

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