Anti-PGRPS抗体[188C424]
参阅全部 PGRPS 一抗
小鼠单克隆抗体[188C424] to PGRPS
Mouse
适用于: IHC-P, WBmore details
与反应: Human
Synthetic peptide: YVLKGHRDVQRTLSPG , corresponding to amino acids 165-180 of Human PGRPS.
IHC: Human esophagus cells. WB: Jurkat cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Protein G purified
单克隆
188C424
IgG1
Abpromise™承诺保证使用ab13903于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
WB | Use a concentration of 2 µg/ml. Predicted molecular weight: 22 kDa. |
Entrez Gene: 8993 Human
Omim: 604963 Human
SwissProt: O75594 Human
Unigene: 137583 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRPS antibody [188C424] (ab13903)
Immunocytochemistry analysis of human esophagus cells labelling PGRPS with ab13903 at 20 µg/mL. The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining of peripheral leukocytes and neutrophils was observed. Staining was performed by Histowiz.
Western blot - Anti-PGRPS antibody [188C424] (ab13903)
Anti-PGRPS antibody [188C424] (ab13903) at 2 µg/ml + Jurkat cell lysate
Predicted band size: 22 kDa
Observed band size: 28 kDawhy is the actual band size different from the predicted?