Anti-5HT3A receptor/5ht3 antibody

• 产品名称

  Anti-5HT3A receptor/5ht3抗体
  参阅全部 5HT3A receptor/5ht3 一抗

• 描述

  兔多克隆抗体to 5HT3A receptor/5ht3

• 宿主

  Rabbit

• 经测试应用

  适用于: IHC-P, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Synthetic peptide corresponding to Rat 5HT3A receptor/5ht3. The immunogen is a mixture of synthetic peptides corresponding to amino acids 27-42 (RATQAHSTTQPALLRL) and 427-442 (LSSIRHSLEKRDEMRE).
  Database link: P35563

• 阳性对照

• human brain lysate

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.4
  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab13897于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 4831 Mouse

• Unigene: 55109 Rat

• Entrez Gene: 3359 Human

• Entrez Gene: 15561 Mouse

• Entrez Gene: 79246 Rat

• Omim: 182139 Human

• SwissProt: P46098 Human

• SwissProt: P23979 Mouse

• SwissProt: P35563 Rat

• Unigene: 413899 Human

• 别名

• 5 HT3A receptor antibody

• 5 HT3RA antibody

• 5 hydroxytryptamine (serotonin) receptor 3A antibody

• 5-HT-3 antibody

• 5-HT3-A antibody

• 5-HT3A antibody

• 5-HT3R antibody

• 5-hydroxytryptamine receptor 3 antibody

• 5-hydroxytryptamine receptor 3A antibody

• 5HT receptor antibody

• 5HT3 serotonin receptor antibody

• 5HT3A_HUMAN antibody

• 5HT3R antibody

• HTR3 antibody

• Htr3a antibody

• Serotonin receptor 3A antibody

• Serotonin-gated ion channel receptor 3a antibody

• Serotonin-gated ion channel receptor antibody

• 5 HT 3 antibody

• 5 HT3 receptor antibody

• 5 HT3A antibody

• 

  Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897)

  All lanes : Anti-5HT3A receptor/5ht3 antibody (ab13897) at 3 µg/ml
  
  Lane 1 : Human Brain tissue lysate
  Lane 2 : Mouse Brain tissue lysate
  Lane 3 : Rat Brain tissue lysate
  
  Predicted band size: 55 kDa
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT3A receptor/5ht3 antibody (ab13897)

  IHC image of ab13897 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13897, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

• 

  Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897)

  Western Blot of human brain lysate staining 5HT3A receptor with ab13897