Anti-TXNDC5抗体
参阅全部 TXNDC5 一抗
山羊多克隆抗体to TXNDC5
Goat
This antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437; NP_071368.
适用于: IHC-P, WB, Flow Cyt (Intra), ICC/IFmore details
与反应: Human
Synthetic peptide corresponding to Human TXNDC5 aa 400 to the C-terminus (C terminal). (NP_001139021.1)
Database link: NP_110437.2
Flow Cyt (Intra): HeLa cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab13820于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). A 1 hour primary incubation is recommended for this product. | |
| Flow Cyt (Intra) | Use a concentration of 10 µg/ml. | |
| ICC/IF | (1) | Use a concentration of 10 µg/ml. |
Entrez Gene: 81567 Human
SwissProt: Q8NBS9 Human
Unigene: 150837 Human
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Western blot - Anti-TXNDC5 antibody (ab13820)
All lanes : Anti-TXNDC5 antibody (ab13820) at 0.1 µg/ml
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : TXNDC5 knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 47 kDawhy is the actual band size different from the predicted?
False colour image of Western blot: Anti-TXNDC5 antibody staining at 0.1 µg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab13820 was shown to bind specifically to TXNDC5. A band was observed at 47 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TXNDC5 knockout cell line ab266609 (knockout cell lysate ab263403). To generate this image, wild-type and TXNDC5 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216778) at 1/20000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TXNDC5 antibody (ab13820)
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation with ab13820 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Flow Cytometry (Intracellular) - Anti-TXNDC5 antibody (ab13820)
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab13820 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-TXNDC5 antibody (ab13820)
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab13820 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Western blot - Anti-TXNDC5 antibody (ab13820)
All lanes : Anti-TXNDC5 antibody (ab13820) at 0.1 µg/ml
Lane 1 : HEK-293 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDa
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