Anti-PRDM1/Blimp1抗体
参阅全部 PRDM1/Blimp1 一抗
山羊多克隆抗体to PRDM1/Blimp1
Goat
适用于: ICC, ChIP, Flow Cytmore details
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog, Pig
Synthetic peptide corresponding to Human PRDM1/Blimp1 aa 800 to the C-terminus (C terminal).
Database link: O75626
ICC: A431 cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab13700于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use a concentration of 10 µg/ml. | |
| ChIP | Use at an assay dependent concentration. PubMed: 17264218 | |
| Flow Cyt | (1) | Use at an assay dependent concentration. ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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SwissProt: Q60636 Mouse
Unigene: 436023 Human
Unigene: 4800 Mouse
Entrez Gene: 639 Human
Entrez Gene: 12142 Mouse
Omim: 603423 Human
SwissProt: O75626 Human
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Immunocytochemistry - Anti-PRDM1/Blimp1 antibody (ab13700)
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
