Anti-TNFAIP3 antibody [59A426]

• 产品名称

  Anti-TNFAIP3抗体[59A426]
  参阅全部 TNFAIP3 一抗

• 描述

  小鼠单克隆抗体[59A426] to TNFAIP3

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt (Intra), IHC-P, WBmore details
  不适用于: ICC

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein corresponding to Human TNFAIP3.
  Database link: P21580

• 表位

  The epitope has been mapped to the C-terminal portion of A20, amino acids 440-790.

• 阳性对照

• WB: Daudi and HeLa cell lysates. Flow Cyt (Intra): HepG2 cells

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  59A426

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab13597于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for ICC.

• 数据库链接

• Entrez Gene: 7128 Human

• Omim: 191163 Human

• SwissProt: P21580 Human

• Unigene: 211600 Human

• 别名

• MGC138687 antibody

• MGC138688 antibody

• OTU domain containing protein 7C antibody

• OTU domain-containing protein 7C antibody

• OTUD7C antibody

• Putative DNA binding protein A20 antibody

• Putative DNA-binding protein A20 antibody

• TNAP3_HUMAN antibody

• TNF alpha-induced protein 3 antibody

• TNFA1P2 antibody

• TNFAIP 3 antibody

• TNFAIP3 (A20) antibody

• TNFAIP3 antibody

• Tumor necrosis factor alpha induced protein 3 antibody

• Tumor necrosis factor alpha-induced protein 3 antibody

• Tumor necrosis factor induced protein 3 antibody

• Tumor necrosis factor inducible protein A20 antibody

• tumor necrosis factor, alpha-induced protein 3 antibody

• Zinc finger protein A20 antibody

• A20 antibody

• AISBL antibody

• MGC104522 antibody

Western blot - Anti-TNFAIP3 antibody [59A426] (ab13597)

All lanes : Anti-TNFAIP3 antibody [59A426] (ab13597) at 1/500 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TNFAIP3 knockout HeLa cell lysate
Lane 3 : Jurkat cell treated with 5ng/ml PMA for 48 hours and then treated with 2µg/ml PHA for 48 hours, whole cell lysate
Lane 4 : Untreated Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Observed band size: 80 kDawhy is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab13597 observed at 80 kDa. Red - loading control, ab181602 observed at 37 kDa.

ab13597 Anti-TNFAIP3 antibody [59A426] was shown to specifically react with TNFAIP3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265983 (knockout cell lysate ab257112) was used. Wild-type and TNFAIP3 knockout samples were subjected to SDS-PAGE. ab13597 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

• 

  Western blot - Anti-TNFAIP3 antibody [59A426] (ab13597)

  Anti-TNFAIP3 antibody [59A426] (ab13597) at 4 µg/ml + Jurkat cell lysate

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [59A426] (ab13597)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue labelling TNFAIP3 with ab13597 at 5µg/ml. Staining was enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

• 

  Western blot - Anti-TNFAIP3 antibody [59A426] (ab13597)

  All lanes : Anti-TNFAIP3 antibody [59A426] (ab13597) at 1 µg/ml
  
  Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Observed band size: 90 kDawhy is the actual band size different from the predicted?
  Additional bands at: 15 kDa, 34 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 20 minutes
  

• 

  Flow Cytometry (Intracellular) - Anti-TNFAIP3 antibody [59A426] (ab13597)

  Overlay histogram showing HepG2 cells stained with ab13597 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13597, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HepG2 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.