Anti-TRF2 antibody [4A794]

• 产品名称

  Anti-TRF2抗体[4A794]
  参阅全部 TRF2 一抗

• 描述

  小鼠单克隆抗体[4A794] to TRF2

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, WB, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length protein corresponding to Human TRF2. Baculovirus expressing full length TRF2 protein. NP_005643
  Database link: Q15554

• 阳性对照

• WB: Jurkat and HeLa cell lysate Flow Cyt (Intra): Jurkat and HeLa cell lysate

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.05% Sodium azide
  Constituents: PBS, 0.05% BSA

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  4A794

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab13579于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7014 Human

• Omim: 602027 Human

• SwissProt: Q15554 Human

• Unigene: 63335 Human

• 别名

• Telomeric repeat binding protein 2 antibody

• Telomeric repeat-binding factor 2 antibody

• TERF 2 antibody

• Terf2 antibody

• TERF2_HUMAN antibody

• TRBF 2 antibody

• TRBF2 antibody

• TRF 2 antibody

• TRF2 antibody

• TTAGGG repeat binding factor 2 antibody

• TTAGGG repeat-binding factor 2 antibody

• Telomeric DNA binding protein antibody

• Telomeric DNA-binding protein antibody

• Telomeric repeat binding factor 2 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)

  ab13579 staining TRF2 in human bladder carcinoma cells using Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigeen retrieval carried out by heat treatment in Citrate buffer pH6.0. Primary antibody diluted to 4μg/ml.

• 

  Western blot - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)

  Anti-TRF2 antibody [4A794] (ab13579) at 2 µg/ml + Jurkat cell lysate
  
  Secondary
  Goat anti-mouse Ig HRP
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)

  ab13579 (1µg/ml) staining TRF2 in human spleen, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the germinal follicle.
  Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  

• 

  Western blot - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)

  Anti-TRF2 antibody [4A794] (ab13579) + HeLa cell lysate
  
  Performed under reducing conditions.
  

• 

  Flow Cytometry (Intracellular) - Anti-TRF2 antibody [4A794] (ab13579)

  Overlay histogram showing HeLA cells stained with ab13579 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13579, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Flow Cytometry (Intracellular) - Anti-TRF2 antibody [4A794] (ab13579)

  Flow Cytometry (Intracellular) analysis of Jurkat cells labeling RF2 with ab13579 (red). The shaded histogram represents cells alone and blue represents isotype control.

   

• 

  Western blot - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)

  Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579.  2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.

  Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579. 2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.