Anti-Dnmt1 antibody [60B1220.1]

• 产品名称

  Anti-Dnmt1抗体[60B1220.1]
  参阅全部 Dnmt1 一抗

• 描述

  小鼠单克隆抗体[60B1220.1] to Dnmt1

• 宿主

  Mouse

• 特异性

  This antibody detects a ~180 kDa protein, corresponding to the apparent molecular mass of Dnmt1 on SDS-PAGE immunoblots in samples of human and mouse origin. Immunogen itself has been shown to be toxic.

• 经测试应用

  适用于: IHC-P, Flow Cyt, WBmore details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Cow

• 免疫原

  Synthetic peptide:

  EKDDREDKENAFKR

  , corresponding to amino acids 637-650 of Human Dnmt1

  Run BLAST with Run BLAST with 

• 阳性对照

• WB: H1299 cell lysate. IHC-Fr: Human colon carcinoma tissue. Mouse medullar kidney tissue. Flow Cyt: HeLa cells.

• 常规说明

  For maximum product recovery, centrifuge the product vial before removing cap. Shipped at on gel packs.

  DNMT1 high levels are toxic, as a result it may be tough to find sometimes. Signal amplification might be needed.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituents: PBS, 50% Glycerol

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 纯化说明

  This antibody is affinity purified.

• 克隆

  单克隆

• 克隆编号

  60B1220.1

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab13537于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 128580 Mouse

• Entrez Gene: 281119 Cow

• Entrez Gene: 1786 Human

• Entrez Gene: 13433 Mouse

• Omim: 126375 Human

• SwissProt: Q24K09 Cow

• SwissProt: P26358 Human

• SwissProt: P13864 Mouse

• Unigene: 202672 Human

• 别名

• CXXC-type zinc finger protein 9 antibody

• CXXC9 antibody

• DNA (cytosine 5 ) methyltransferase 1 antibody

• DNA (cytosine-5)-methyltransferase 1 antibody

• DNA methyltransferase 1 antibody

• DNA methyltransferase HsaI antibody

• DNA methyltransferase M.HsaI. antibody

• DNA MTase antibody

• DNA MTase HsaI antibody

• DNMT 1 antibody

• DNMT antibody

• Dnmt1 antibody

• DNMT1_HUMAN antibody

• Dnmt1o antibody

• FLJ16293 antibody

• HSN1E antibody

• M.HsaI antibody

• MCMT antibody

• Met1 antibody

• MGC104992 antibody

• mMmul antibody

• MommeD2 antibody

• ADCADN antibody

• AIM antibody

• CXXC finger protein 9 antibody

• 

  Western blot - Anti-Dnmt1 antibody [60B1220.1] (ab13537)

  Anti-Dnmt1 antibody [60B1220.1] (ab13537) at 1/1000 dilution + H1299 (Human non-small cell lung carcinoma cell line) lysate
  
  Predicted band size: 180 kDa
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [60B1220.1] (ab13537)

  Immunohistochemistry analysis of formalin-fixed human colon carcinoma tissue labelling Dnmt1 with ab13537 at 1/10000 dilution (12 hours at 4°C), followed by biotin Goat Anti-Mouse antibody at 1/2000 for 1 hour at RT. Counterstain: Hematoxylin (purple/blue) nuclear stain at 200µl for 2 mins at RT.

• 

  Flow Cytometry - Anti-Dnmt1 antibody [60B1220.1] (ab13537)

  Overlay histogram showing HeLa cells stained with ab13537 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13537, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [60B1220.1] (ab13537)

  Immunohistochemistry analysis of mouse medullar kidney tissue labelling Dnmt1 with ab13537 at 1/10000 dilution, followed by biotin Goat Anti-Mouse antibody (brown). Counterstain: Hematoxylin (purple/blue) nuclear stain.

• 

  Western blot - Anti-Dnmt1 antibody [60B1220.1] (ab13537)This image is courtesy of an anonymous Abreview

  All lanes : Anti-Dnmt1 antibody [60B1220.1] (ab13537) at 1 µg/ml
  
  Lane 1 : HCT116 whole cell lysate
  Lane 2 : HCT116 DNMT1 KO whole cell lysate
  Lane 3 : HCT116 DNMT3b KO whole cell lysate
  
  Secondary
  All lanes : HRP conjugated Sheep anti-mouse IgG
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 180 kDa
  
  
  Exposure time: 1 minute
  

  See Abreview