Anti-SOD2/MnSOD抗体
参阅全部 SOD2/MnSOD 一抗
兔多克隆抗体to SOD2/MnSOD
Rabbit
适用于: WBmore details
与反应: Mouse, Rat
预测可用于: Human
Recombinant fragment corresponding to Human SOD2/MnSOD.
Database link: P04179
WB: Mouse Brain ; Rat Brain ; Rat Brain Membrane; Mouse Liver ; Mouse Hepatocytes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
This antibody was immunoaffinity purified.
多克隆
IgG
Abpromise™承诺保证使用ab13533于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (22) | 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa). |
hide
Unigene: 290876 Mouse
Unigene: 10488 Rat
Entrez Gene: 6648 Human
Entrez Gene: 20656 Mouse
Omim: 147460 Human
SwissProt: P04179 Human
SwissProt: P09671 Mouse
Unigene: 487046 Human
Manganese containing superoxide dismutase antibody
Manganese SOD antibody
Manganese superoxide dismutase antibody
Mangano superoxide dismutase antibody
Mn SOD antibody
Mn superoxide dismutase antibody
MNSOD antibody
MVCD6 antibody
SOD 2 antibody
SOD2 antibody
SODM_HUMAN antibody
Superoxide dismutase [Mn] mitochondrial antibody
Superoxide dismutase [Mn], mitochondrial antibody
Superoxide dismutase 2 mitochondrial antibody
Indophenoloxidase B antibody
IPO B antibody
IPOB antibody
Western blot - Anti-SOD2/MnSOD antibody (ab13533)
All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/1000 dilution (o/n at 4degC)
Lane 1 : Mouse brain
Lane 2 : Rat brain
Lane 3 : Rat brain membrane
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP for 1 hr at RT with shaking at 1/5000 dilution
Predicted band size: 26.6 kDa
Block: 5% Skim Milk powder in TBST.
Color Development: Chemiluminescent for HRP for 5 min at RT.
Western blot - Anti-SOD2/MnSOD antibody (ab13533)Image courtesy of anonymous abreviewer
All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution
Lane 1 : mouse liver whole cell lysate
Lane 2 : mouse hepatocytes whole cell lysate
Secondary
All lanes : Goat anti-rabbit HRP conjugate at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26.6 kDa
Observed band size: 24 kDawhy is the actual band size different from the predicted?
Exposure time: 15 seconds
Western blot - Anti-SOD2/MnSOD antibody (ab13533)This image is courtesy of an Abreview submitted by Dr sandra sobocanec
All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution
Lane 1 : 2ug mouse liver homogenate supernatant
Lane 2 : 1ug mouse liver homogenate supernatant
Lane 3 : 0.5ug mouse liver homogenate supernatant
Secondary
All lanes : HRP conjugated donkey anti-rabbit antibody
Performed under reducing conditions.
Predicted band size: 26.6 kDa
Observed band size: 25 kDawhy is the actual band size different from the predicted?
Exposure time: 10 seconds
抱歉，暂无浏览记录
