Anti-LAMP2 antibody [GL2A7]

• 产品名称

  Anti-LAMP2抗体[GL2A7]
  参阅全部 LAMP2 一抗

• 描述

  大鼠单克隆抗体[GL2A7] to LAMP2

• 宿主

  Rat

• 经测试应用

  适用于: IHC-Fr, ICC, ICC/IF, IHC-FoFr, IP, Flow Cyt, WB, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Rabbit, Human
  

• 免疫原

  Tissue, cells or virus corresponding to Mouse LAMP2. Purified preparation of mouse liver lysosomal membranes.

• 阳性对照

• WB: HEK-293 and NIH/3T3 cell lysate.

• 常规说明

  For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituents: PBS, 50% Glycerol

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  GL2A7

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab13524于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3920 Human

• Entrez Gene: 16784 Mouse

• Omim: 309060 Human

• SwissProt: P13473 Human

• SwissProt: P17047 Mouse

• Unigene: 496684 Human

• Unigene: 486 Mouse

• 形式

  Alternative splicing produces 3 isoforms.

• 别名

• LAMP 2 antibody

• LAMP 2C antibody

• LAMP-2 antibody

• LAMP2 antibody

• LAMP2_HUMAN antibody

• LAMPB antibody

• LGP 96 antibody

• LGP110 antibody

• LGP96 antibody

• Lysosomal associated membrane protein 2 antibody

• Lysosome associated membrane glycoprotein 2 antibody

• Lysosome associated membrane protein 2 antibody

• Lysosome-associated membrane glycoprotein 2 antibody

• Lysosome-associated membrane protein 2 antibody

• MAC3 antibody

• CD107 antigen like family member B antibody

• CD107 antigen-like family member B antibody

• CD107b antibody

• 

  Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)

  All lanes : Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/500 dilution (1 hr at RT)
  
  Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 20 µg
  Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) lysate at 10 µg
  
  Secondary
  All lanes : HRP Goat Anti-Rat, 1 hour at RT at 1/100 dilution
  
  
  

  Block: 5% milk + TBST for 1 hour at RT.

• 

  Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)This image is courtesy of an anonymous Abreview.

  Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/1000 dilution + HEK293 cell lysate at 20 µg
  
  Secondary
  Goat anti-rat HRP at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Observed band size: 105 kDawhy is the actual band size different from the predicted?
  Additional bands at: 28 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)
  
  
  Exposure time: 3 minutes
  
  
  

  Blocked with 5% milk for 1 hour at RT.

  Incubated with primary antibody in 5% BSA/TBST for 16 hours at 4°C.

  See Abreview