Anti-ERp57 antibody [MaP.Erp57]

• 产品名称

  Anti-ERp57抗体[MaP.Erp57]
  参阅全部 ERp57 一抗

• 描述

  小鼠单克隆抗体[MaP.Erp57] to ERp57

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, WB, ICC/IF, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Recombinant full length protein (Human).

• 阳性对照

• WB: HAP1, HepG2 and HEK-293 whole cell lysate; mouse pancreatic cancer cell lysate. ICC/IF: HeLa and HaCaT cells. IHC-P: Muse backskin tissue. IHC-Fr: Mouse duodenum, pancreas, and pancreatic neoplastic tissue. Flow Cyt: HeLa cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituents: PBS, 50% Glycerol

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 纯化说明

  This antibody is affinity purified.

• 克隆

  单克隆

• 克隆编号

  MaP.Erp57

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab13506于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 2923 Human

• Entrez Gene: 14827 Mouse

• Omim: 602046 Human

• SwissProt: P30101 Human

• SwissProt: P27773 Mouse

• Unigene: 591095 Human

• Unigene: 263177 Mouse

• 别名

• Disulfide isomerase ER 60 antibody

• Disulfide isomerase ER-60 antibody

• Endoplasmic reticulum resident protein 57 antibody

• Endoplasmic reticulum resident protein 60 antibody

• ER p57 antibody

• ER protein 57 antibody

• ER protein 60 antibody

• ERp 57 antibody

• ERp57 antibody

• ERp60 antibody

• ERp61 antibody

• Glucose Regulated Protein 58 Kd antibody

• GRP 57 antibody

• GRP 58 antibody

• GRP57 antibody

• HsT17083 antibody

• p58 antibody

• PDIA 3 antibody

• PDIA3 antibody

• PDIA3_HUMAN antibody

• Phospholipase C alpha antibody

• PI PLC antibody

• Protein disulfide isomerase A3 antibody

• Protein disulfide isomerase family A member 3 antibody

• Protein disulfide-isomerase A3 antibody

• 58 kDa glucose regulated protein antibody

• 58 kDa glucose-regulated protein antibody

• 58 kDa microsomal protein antibody

• 

  Western blot - Anti-ERp57 antibody [MaP.Erp57] (ab13506)

  Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
  Lane 2: ERp57 knockout HAP1 whole cell lysate (20 µg)
  Lane 3: HepG2 whole cell lysate (20 µg)
  Lane 4: HEK293 whole cell lysate (20 µg)

  Lanes 1 - 4: Merged signal (red and green). Green - ab13506 observed at 57 kDa. Red - loading control, ab181602, observed at 37 kDa.

  ab13506 was shown to specifically react with ERp57 in wild-type HAP1 cells as signal was lost in ERp57 knockout cells. Wild-type and ERp57 knockout samples were subjected to SDS-PAGE. ab13506 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 0.5 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [MaP.Erp57] (ab13506)

  ICC/IF image of ab13506 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab13506 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Western blot - Anti-ERp57 antibody [MaP.Erp57] (ab13506)This image is courtesy of an Abreview submitted by Pawel Mazur

  ERp57 antibody (ab13506) at 1/1000 dilution (in PBST) for 8 hours at 4°C + lysate of mouse of pancreatic cancer cell line at 25 µg.
  
  Secondary
  An HRP conjugated Sheep anti-mouse IgG polyclonal at 1/5000 dilution
  developed using the ECL technique
  
  Performed under non-reducing conditions.
  
  Exposure time: 1 minute
  
  Blocking Step: 10% Milk for 1 hour at room temperature.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [MaP.Erp57] (ab13506)

  Paraffin-embedded mouse backskin tissue ficed with Bouin's fixative, stained for ERp57 using ab13506 at 1/100 dilution in ICC/IF. Primary antibody was incubated for 1 hour at room temperature. Secondary antibody is a FITC-conjugated goat anti-mouse (green) incubated at 1/50 dilution for 1 hour at room temperature.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [MaP.Erp57] (ab13506)

  HaCaT (Human keratinocyte cell line) cells labeling ERp57 using ab13506 at 1/100 dilution in ICC.IF. Cells were fixed using 100% methanol for 10 minutes at -20°C and incubated with primary antibody for 1 hour at room temperature. Secondary antibody is a FITC-conjugated goat anti-mouse (green) incubated at 1/50 dilution for 1 hour at room temperature.

   

• 

  Flow Cytometry - Anti-ERp57 antibody [MaP.Erp57] (ab13506)

  Overlay histogram showing HeLa cells stained with ab13506 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13506, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.