Anti-Alpha B Crystallin antibody [1B6.1-3G4]

• 产品名称

  Anti-Alpha B Crystallin抗体[1B6.1-3G4]
  参阅全部 Alpha B Crystallin 一抗

• 描述

  小鼠单克隆抗体[1B6.1-3G4] to Alpha B Crystallin

• 宿主

  Mouse

• 经测试应用

  适用于: WB, Flow Cyt, IHC-Pmore details

• 种属反应性

  与反应: Cow, Human
  预测可用于: Mouse, Rat, Chicken

• 免疫原

  Full length native protein (purified) corresponding to Cow Alpha B Crystallin.
  Database link: P02510

• 阳性对照

• Recombinant Bovine alpha B Crystallin Protein.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.2
  Preservative: 0.09% Sodium azide
  Constituents: 49.91% PBS, 50% Glycerol (glycerin, glycerine)

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  1B6.1-3G4

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab13496于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 396089 Chicken

• Entrez Gene: 281719 Cow

• Entrez Gene: 1410 Human

• Entrez Gene: 12955 Mouse

• Entrez Gene: 25420 Rat

• Omim: 123590 Human

• SwissProt: Q05713 Chicken

• SwissProt: P02510 Cow

• SwissProt: P02511 Human

• SwissProt: P23927 Mouse

• SwissProt: P23928 Rat

• Unigene: 53454 Human

• Unigene: 178 Mouse

• Unigene: 98208 Rat

别名

• AACRYA antibody

• Alpha B crystallin antibody

• Alpha crystallin B chain antibody

• Alpha(B)-crystallin antibody

• Alpha-crystallin B chain antibody

• CRYA2 antibody

• Cryab antibody

• CRYAB_HUMAN antibody

• Crystallin alpha B antibody

• Crystallin alpha polypeptide 2 antibody

• CTPP2 antibody

• Heat shock 20 kD like protein antibody

• Heat shock protein beta 5 antibody

• Heat shock protein beta-5 antibody

• HspB5 antibody

• Renal carcinoma antigen NY REN 27 antibody

• Renal carcinoma antigen NY-REN-27 antibody

• Rosenthal fiber component antibody

• 

  Flow Cytometry - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

  Overlay histogram showing SH-SY5Y cells stained with ab13496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13496, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  This image was generated using the ascites version of the product.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

  Immunohistochemistry (paraffin sections) analysis of Human heart tissue labelling Alpha B Crystallin with ab13496 at 20 µg/mL

• 

  Western blot - Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496)

  All lanes : Anti-Alpha B Crystallin antibody [1B6.1-3G4] (ab13496) at 1/1000 dilution
  
  Lane 2 : a-Crystallin (bovine), (native)
  Lane 3 : aB-Crystallin (bovine), (native)
  
  Predicted band size: 21 kDa
  Observed band size: 20 kDawhy is the actual band size different from the predicted?