Anti-SP1抗体
参阅全部 SP1 一抗
兔多克隆抗体to SP1
Rabbit
适用于: IP, WB, IHC-Pmore details
与反应: Human
预测可用于: Rabbit, Goat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Zebra finch, Xenopus tropicalis
Synthetic peptide corresponding to Human SP1 (C terminal).
Database link: P08047
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.764% Sodium citrate, 1.815% Tris, 0.88% Sodium chloride, 0.07% Sodium hydroxide, 0.27% Phosphoric acid
pH 7 to 8.
浓度
50 µl 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab13370于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (1) | Use at 1-4 µg/mg of lysate. |
| WB | (8) | 1/2500 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
| IHC-P | 1/5000. |
Entrez Gene: 395303 Chicken
Entrez Gene: 451939 Chimpanzee
Entrez Gene: 6667 Human
Entrez Gene: 100440745 Orangutan
Entrez Gene: 702710 Rhesus monkey
Entrez Gene: 431939 Xenopus laevis
Entrez Gene: 394744 Xenopus tropicalis
Omim: 189906 Human
SwissProt: P08047 Human
Unigene: 620754 Human
Unigene: 649191 Human
SP 1 antibody
SP1 antibody
Sp1 transcription factor antibody
SP1_HUMAN antibody
Specificity protein 1 antibody
Transcription factor Sp1 antibody
TSFP 1 antibody
TSFP1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (ab13370)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue sections labeling SP1 with ab13370 at 1/5000 dilution. DAB detection.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (ab13370)ab13370 (2µg/ml) staining SP1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunoprecipitation - Anti-SP1 antibody (ab13370)
SP1 was immunoprecipitated from HeLa cells whole cell lysate with ab13370. Anti-SP1 antibody - ChIP Grade (ab13370) used at 6µg per reaction. SP1 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated SP1, ab13370 was used at 1µg/ml. Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (ab13370)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling SP1 with ab13370 at 1/5000 dilution. DAB detection.
Western blot - Anti-SP1 antibody (ab13370)
Detection of Human SP1 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate (50 mcg for E; 10 mcg for T) from HEK 293T cells that were mock transfected (E) or transfected with a Sp1 expression construct (T). B. Whole cell lysate (700 mcg) from HEK 293T cells. Antibody: A. Affinity purified rabbit anti-Sp1 antibody (ab13370) used at the indicated concentrations. B. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibodies (lane 2: ab13405, lane 3: ab13370; lane 1: SP-1 antibody, details unknown) using each antibody at 3.75 mcg/mg lysate. Subsequently, ab13370 was used at 0.04 mcg/ml for WB. Detection: Chemiluminescence with an exposure time of 10 seconds (A) or 1 min (B).
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