Anti-RPS6 (phospho S235 + S236) antibody

• 产品名称

  Anti-RPS6 (phospho S235 + S236)抗体
  参阅全部 RPS6 一抗

• 描述

  兔多克隆抗体to RPS6 (phospho S235 + S236)

• 宿主

  Rabbit

• 特异性

  This antibody does not cross react with RPS6 phosphorylated on serines 244 and 247.

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• HeLa +/- TNF-alpha or anisomycin

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.30
  Preservative: 0.05% Sodium azide
  Constituents: PBS, 50% Glycerol, 0.1% BSA
  
  Dulbecco's PBS without magnesium and calcium

• 浓度

• 50 µl 浓度为 0.1 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated RPS6. The final product is generated by affinity chromatography using a RPS6-derived peptide that is phosphorylated at serines 235 and 236.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab12864于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 6194 Human

• Entrez Gene: 20104 Mouse

• Entrez Gene: 667739 Mouse

• Entrez Gene: 29304 Rat

• Omim: 180460 Human

• SwissProt: P62753 Human

• SwissProt: P62754 Mouse

• SwissProt: P62755 Rat

• Unigene: 408073 Human

• Unigene: 379007 Mouse

• Unigene: 425090 Mouse

• Unigene: 34341 Rat

别名

• 40S ribosomal protein S6 antibody

• Air8 antibody

• NP33 antibody

• Phosphoprotein NP33 antibody

• Pp30 antibody

• Ribosomal protein S6 antibody

• RP S6 antibody

• rps6 antibody

• RS6 antibody

• RS6_HUMAN antibody

• S6 antibody

• S6 Ribosomal Protein antibody

• 

  Western blot - Anti-RPS6 (phospho S235 + S236) antibody (ab12864)

  Peptide Competition.
  Lysates prepared from HeLa cells left untreated (1) or treated with anisomycin (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room  temperature, and incubated with ab12864 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with a goat anti-rabbit IgG HRP conjugate secondary antibody and bands were detected using the Pierce SuperSignalTM method. The data show that only the peptide corresponding to RPS6 blocks the signal, verifying the specificity of the antibody. Peptide Competition. Lysates prepared from HeLa cells left untreated (1) or treated with anisomycin (2-5) were resolved by SDS-PAGE on a 14% po