Anti-Engrailed and Invected antibody [4D9]

• 产品名称

  Anti-Engrailed and Invected抗体[4D9]

• 描述

  小鼠单克隆抗体[4D9] to Engrailed and Invected

• 宿主

  Mouse

• 经测试应用

  适用于: IHC - Wholemountmore details

• 种属反应性

  与反应: Drosophila melanogaster
  预测可用于: Chicken, Xenopus laevis, Zebrafish, Crayfish不与反应: Mouse

• 免疫原

  This product was produced with the following immunogens:
  Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
  
  Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

• 表位

  Epitope has been localized to residues 38-58 of the homeodomain.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 10 µg 浓度为 0.9 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  4D9

• 骨髓瘤

  NS1

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab12454于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 36239 Drosophila melanogaster

• Entrez Gene: 36240 Drosophila melanogaster

• SwissProt: P02836 Drosophila melanogaster

• SwissProt: P05527 Drosophila melanogaster

• 别名

• Engrailed/Invected antibody

• Homeobox protein invected antibody

• Inv antibody

• Invected antibody

• En antibody

• Eng antibody

• Engrailed antibody

• 

  IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454)This image is courtesy of Dr Torsten Bossing at the University of Plymouth.

  ab12454 used in immunohistochemistry at 1/200 on Drosophila embryos. The protocol used is available here.

  Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with DAB.

• 

  IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454)This image is courtesy of Dr Torsten Bossing at the University of Plymouth.

  ab12454 used in immunofluorescence at 1/200 on Drosophila embryos. The protocol used is available here.

  Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining.

• 

  IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454)This image is courtesy of Dr Torsten Bossing at the University of Plymouth.

  ab12454 used in immunohistochemistry (Wholemount) at 1/200 dilution on Drosophila embryos. The protocol used is available here.

  Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with DAB.