Anti-Syntaxin 2/STX2抗体
参阅全部 Syntaxin 2/STX2 一抗
兔多克隆抗体to Syntaxin 2/STX2
Rabbit
适用于: ICC/IF, WBmore details
与反应: Human
Synthetic peptide corresponding to Rat Syntaxin 2/STX2 aa 1-100 conjugated to keyhole limpet haemocyanin.
Database link: P50279
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
This antibody was purified on a peptide bound immunoaffinity column.
多克隆
IgG
Abpromise™承诺保证使用ab12369于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33.3 kDa). human and 1/1000-1/2000 for other species. |
Entrez Gene: 2054 Human
Omim: 132350 Human
SwissProt: P32856 Human
Unigene: 437585 Human
Inhibition of syntaxin-2 and Vamp8 function by overexpression of nonmembrane-anchored mutants caused failure of cytokinesis leading to the formation of binucleated cells. There are 3 isoforms produced by alternative splicing.
MGC51014 antibody
OTTHUMP00000238636 antibody
OTTHUMP00000238637 antibody
Stx2 antibody
STX2_HUMAN antibody
STX2A antibody
STX2B antibody
STX2C antibody
Syntaxin 2A antibody
Syntaxin 2B antibody
Syntaxin 2C antibody
Syntaxin-2 antibody
EPIM antibody
Epimorphin antibody
EPM antibody
Western blot - Anti-Syntaxin 2/STX2 antibody (ab12369)
All lanes : Anti-Syntaxin 2/STX2 antibody (ab12369) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : Human skin tissue lysate - total protein (ab30166)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33.3 kDa
Observed band size: 33.3 kDa
Additional bands at: 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 2/STX2 antibody (ab12369)
ICC/IF image of ab12369 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12369, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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