Anti-Hsp27 antibody

• 产品名称

  Anti-Hsp27抗体
  参阅全部 Hsp27 一抗

• 描述

  兔多克隆抗体to Hsp27

• 宿主

  Rabbit

• 特异性

  This antibody detects an 25 kDa protein, corresponding to the apparent molecular mass of Heat Shock Protein 27 (Hsp27) on SDS-PAGE immunoblots. This antibody has been shown to react with both the phosphorylated and the non-phosphorylated forms of Hsp27. Note: It has been reported that certain murine cell lines do not express Hsp27 under certain conditions. This antibody also recognizes a mitochondrial small Hsp (35 kDa) in rat PC12 cells. This mitochondrial small Hsp is proposed to protect mitochondrial complex I from oxidative stress.

• 经测试应用

  适用于: IHC, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  预测可用于: Pig, Monkey

• 免疫原

  Recombinant full length protein corresponding to Human Hsp27.
  Database link: P04792

• 常规说明

  For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituents: PBS, 50% Glycerol (glycerin, glycerine)

• 纯度

  Protein A purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab12351于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 3315 Human

• Entrez Gene: 15507 Mouse

• Entrez Gene: 493184 Pig

• Entrez Gene: 24471 Rat

• Omim: 602195 Human

• SwissProt: P04792 Human

• SwissProt: P14602 Mouse

• SwissProt: Q5S1U1 Pig

• SwissProt: P42930 Rat

• Unigene: 520973 Human

• Unigene: 13849 Mouse

• Unigene: 3841 Rat

别名

• Heat shock 27kDa protein antibody

• 28 kDa heat shock protein antibody

• CMT2F antibody

• DKFZp586P1322 antibody

• epididymis secretory protein Li 102 antibody

• Estrogen regulated 24 kDa protein antibody

• Estrogen-regulated 24 kDa protein antibody

• Heat shock 25kDa protein 1 antibody

• Heat shock 27 kDa protein antibody

• Heat shock 27kD protein 1 antibody

• Heat shock 27kDa protein 1 antibody

• Heat shock 28kDa protein 1 antibody

• Heat Shock Protein 27 antibody

• Heat shock protein beta 1 antibody

• Heat shock protein beta-1 antibody

• heat shock protein family B (small) member 1 antibody

• HEL-S-102 antibody

• HMN2B antibody

• HS.76067 antibody

• Hsp 25 antibody

• HSP 27 antibody

• Hsp 28 antibody

• Hsp B1 antibody

• Hsp25 antibody

• HSP27 antibody

• Hsp28 antibody

• HspB1 antibody

• HSPB1_HUMAN antibody

• SRP27 antibody

• Stress responsive protein 27 antibody

• Stress-responsive protein 27 antibody

• 

  Western blot - Anti-Hsp27 antibody (ab12351)

  All lanes : Anti-Hsp27 antibody (ab12351) at 1 µg/ml
  
  Lane 1 : MW Marker
  Lane 2 : HSP27 Recombinant Human Protein
  Lane 3 : HSP25 Recombinant Murine Protein (Negative Control)
  Lane 4 : HeLa cell lysate
  Lane 5 : HeLa cell lysate, Heat Shocked
  Lane 6 : Vero, Heat Shocked
  Lane 7 : 3T3 cell lysate, heat shocked
  Lane 8 : PC-12 cell lysate, Heat Shocked
  
  Predicted band size: 25 kDa
  
  

• 

  Immunohistochemistry - Anti-Hsp27 antibody (ab12351)

  Immunohistochemical analysis of human heart tissue sections with ab12351 at 10 µg/mL.

• 

  Western blot - Anti-Hsp27 antibody (ab12351)This image is courtesy of an anonymous Abreview

  Anti-Hsp27 antibody (ab12351) at 1/500 dilution + Rat keratinocytes, whole cell lysate at 10 µg
  
  Secondary
  HRP conjugated goat anti-rabbit antibody
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 25 kDa
  Observed band size: 25 kDa
  Additional bands at: 120 kDa (possible non-specific binding), 215 kDa (possible non-specific binding)
  
  
  Exposure time: 5 minutes
  

  See Abreview

• 

  Western blot - Anti-Hsp27 antibody (ab12351)

  All lanes : Anti-Hsp27 antibody (ab12351) at 1/1000 dilution
  
  Lane 1 : Molecular weight marker
  Lane 2 : Cell lysates prepared from untreated PC-12 cells
  Lane 3 : Cell lysates prepared from heat shock treated PC-12 cells
  
  Predicted band size: 25 kDa