Anti-FHL2抗体
参阅全部 FHL2 一抗
兔多克隆抗体to FHL2
Rabbit
适用于: IP, ICC/IF, IHC-P, WBmore details
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to Human FHL2.
Database link: Q14192
WB: HeLa, TCMK-1 and NIH/3T3 whole cell lysates. IP: HeLa cells. IHC-P: Human breast and ovarian carcinoma tissue.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.815% Tris, 1.764% Sodium citrate
浓度
50 µl 浓度为 1 mg/ml
Immunogen affinity purified
This antibody is affinity purified.
多克隆
IgG
Abpromise™承诺保证使用ab12328于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use a concentration of 1 µg/ml. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 44.6 kDa). |
Entrez Gene: 2274 Human
Entrez Gene: 14200 Mouse
Omim: 602633 Human
SwissProt: Q14192 Human
SwissProt: O70433 Mouse
Unigene: 443687 Human
Unigene: 6799 Mouse
Unigene: 3849 Rat
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Western blot - Anti-FHL2 antibody (ab12328)
All lanes : Anti-FHL2 antibody (ab12328) at 0.1 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 44.6 kDa
Exposure time: 30 seconds
Immunoprecipitation - Anti-FHL2 antibody (ab12328)
FHL2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab12328 at 3 µg/mg lysate (different LOT numbers). Western blot was performed from the immunoprecipitate using ab12328 at 1 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL2 antibody (ab12328)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling FHL2 with ab12328 at 1/2000 (0.5 µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL2 antibody (ab12328)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FHL2 with ab12328 at 1/1000 (1µg/ml). Detection: DAB.
Immunocytochemistry/ Immunofluorescence - Anti-FHL2 antibody (ab12328)
ICC image of ab12328 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12328, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
