Anti-FHL2 antibody

• 产品名称

  Anti-FHL2抗体
  参阅全部 FHL2 一抗

• 描述

  兔多克隆抗体to FHL2

• 宿主

  Rabbit

• 特异性

  Reactivity with mouse FHL2 is unlikely owing to divergence of this ortholog from the peptide used for antibody production. The epitope recognized by ab1237 maps to a region between residues 175 and 225 of human Four and a Half LIM domains 2 using the numbering given in entry NP_963849.1 (GeneID 2274).

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan

• 免疫原

  Synthetic peptide corresponding to Human FHL2 aa 175-225.
  Database link: Q14192
  (Peptide available as ab119360)

• 阳性对照

• WB: U-2 OS and HeLa cell lysates; HeLa nuclear extract.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7
  Preservative: 0.1% Sodium azide
  Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris

• 纯度

  Immunogen affinity purified

• 纯化说明

  This antibody is affinity purified.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab12327于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 2274 Human

• Omim: 602633 Human

• SwissProt: Q14192 Human

• Unigene: 443687 Human

• 别名

• Down regulated in rhabdomyosarcoma LIM protein antibody

• Downregulated in rhabdomyosarcoma LIM protein antibody

• DRAL antibody

• FHL 2 antibody

• FHL-2 antibody

• Fhl2 antibody

• FHL2 protein antibody

• FHL2_HUMAN antibody

• Four and a half LIM domain protein 2 antibody

• Four and a half LIM domains 2 antibody

• Four and a half LIM domains protein 2 antibody

• KIAA0990 antibody

• LIM domain protein DRAL antibody

• Skeletal muscle LIM protein 3 antibody

• Skeletal muscle LIM-protein 3 antibody

• SLIM 3 antibody

• SLIM-3 antibody

• SLIM3 antibody

• AAG 11 antibody

• AAG11 antibody

• Aging associated gene 11 antibody

• 

  Western blot - Anti-FHL2 antibody (ab12327)

  All lanes : Anti-FHL2 antibody (ab12327) at 1/1000 dilution
  
  Lane 1 : Wild-type U-2 OS cell lysate
  Lane 2 : FHL2 knockout U-2 OS cell lysate
  Lane 3 : HeLa cell lysate
  
  Lysates/proteins at 40 µg per lane.
  
  Performed under reducing conditions.
  
  Predicted band size: 32 kDa
  Observed band size: 32 kDa
  
  
  
  

  Lanes 1 - 3: Merged signal (red and green). Green - ab12327 observed at 32 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

  ab12327 was shown to react with FHL2 in wild-type U-2 OS cells in western blot with loss of signal observed in FHL2 knockout sample. Wild-type and FHL2 knockout U-2 OS cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab12327 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

• 

  Western blot - Anti-FHL2 antibody (ab12327)

  Samples:

  Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes. Samples: Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.