Anti-PLK1 (phospho T210)抗体
参阅全部 PLK1 一抗
兔多克隆抗体to PLK1 (phospho T210)
Rabbit
Detects a band at 55kDa in mouse A20 cells that we believe corresponds to Plk1 (predicted molecular weight 68kDa). Also gives positive staining in immunohistochemistry in human colon carcinoma as expected. However, we have been unable to conclusively demonstrate the specificity of the antibody for phospho T210 Plk1.
Anti-Polo-like Kinase pT210 Antibody is directed against human phosphorylated Plk1 protein. This antibody is specific for phosphorylated human Plk-1 protein at the pT210 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Plk-1 homologues from human, chimpanzee, pig, chicken, mouse, rat, Xenopus, dog, mosquito, zebra fish, starfish, sea urchin and fruit fly. Cross reactivity with Plk-1 protein homologues from C.elgans and honeybee may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Plk-1 protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.
适用于: WB, IHC-Pmore details
与反应: Mouse, Human
预测可用于: Rat, Xenopus laevis, Drosophila melanogaster, Zebrafish
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
100 µg 浓度为 1 mg/ml
Affinity purified
Anti-Polo-like Kinase pT210 Antibody is directed against human phosphorylated Plk1 protein. This antibody was affinity purified.
多克隆
IgG
Abpromise™承诺保证使用ab12157于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | 1/200 - 1/2000. Predicted molecular weight: 68 kDa. |
| IHC-P | Use at an assay dependent concentration. |
Entrez Gene: 5347 Human
Entrez Gene: 18817 Mouse
Entrez Gene: 380481 Xenopus laevis
Omim: 602098 Human
SwissProt: P53350 Human
SwissProt: Q07832 Mouse
SwissProt: P70032 Xenopus laevis
SwissProt: Q4KMI8 Zebrafish
Unigene: 592049 Human
Unigene: 16525 Mouse
Unigene: 11034 Rat
Unigene: 28141 Xenopus laevis
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Serine/threonine protein kinase PLK1 antibody
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Western blot - Anti-PLK1 (phospho T210) antibody (ab12157)
Anti-PLK1 (phospho T210) antibody (ab12157) at 1/500 dilution + Mouse A20 cell lysate
Predicted band size: 68 kDa
Observed band size: 55 kDawhy is the actual band size different from the predicted?
Additional bands at: 23 kDa, 40 kDa, 42 kDa. We are unsure as to the identity of these extra bands.
We believe the 55kDa band represents Plk1. However, we cannot be sure that the antibody is detecting phospho T210 Plk1 specifically.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody (ab12157)
ab12157 was used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded. Detection with AEC (pink), nuclear counterstaining with Haematoxylin (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody (ab12157)
ab12157 (1/200) detecting Plk1 (phospho T210) in formalin-fixed paraffin embedded human breast carcinoma tissue.
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