Anti-Podoplanin / gp36 antibody [RTD4E10] - BSA and Azide free

• 产品名称

  Anti-Podoplanin抗体[RTD4E10] - BSA and Azide free
  参阅全部 Podoplanin 一抗

• 描述

  叙利亚仓鼠单克隆抗体[RTD4E10] to Podoplanin - BSA and Azide free

• 宿主

  Syrian hamster

• 特异性

  The monoclonal antibody will detect podoplanin on the surface of mouse lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry. This antibody is designed to detect podoplanin, which is also referred to as gp38 (mouse and rat) and gp36 (human).

• 经测试应用

  适用于: IHC-Pmore details

• 种属反应性

  与反应: Mouse
  不与反应: Human

• 免疫原

  Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• Mouse normal skin FFPE tissue sections (IHC-P)

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Constituent: PBS

• 无载体

  是

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 纯化说明

  Purified from cell culture supernatant.

• 克隆

  单克隆

• 克隆编号

  RTD4E10

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11936于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 14726 Mouse

• SwissProt: Q62011 Mouse

• Unigene: 2976 Mouse

• 别名

• Glycoprotein 36 antibody

• gp 36 antibody

• GP 38 antibody

• GP 40 antibody

• gp36 antibody

• GP38 antibody

• GP40 antibody

• HT1A 1 antibody

• HT1A-1 antibody

• HT1A1 antibody

• hT1alpha 1 antibody

• hT1alpha 2 antibody

• hT1alpha1 antibody

• hT1alpha2 antibody

• Lung type I cell membrane associated glycoprotein antibody

• Lung type I cell membrane associated glycoprotein isoform a antibody

• Lung type I cell membrane associated glycoprotein T1A 2 antibody

• OTS 8 antibody

• OTS8 antibody

• OTTHUMP00000009640 antibody

• OTTHUMP00000044504 antibody

• PA2.26 antibody

• PA2.26 antigen antibody

• Pdpn antibody

• PDPN_HUMAN antibody

• Podoplanin antibody

• PSEC0003 antibody

• PSEC0025 antibody

• T1 alpha antibody

• T1 ALPHA GENE antibody

• T1-alpha antibody

• T1A 2 antibody

• T1A antibody

• T1A-2 antibody

• T1A2 antibody

• TI1A antibody

• TIA 2 antibody

• TIA2 antibody

• Aggrus antibody

• D2-40 antibody

• Glycoprotein 36 KD antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Syrian hamster monoclonal [RTD4E10] to Podoplanin - BSA and Azide free (ab11936)

  IHC image of Podoplanin staining in a section of formalin-fixed paraffin-embedded normal mouse skin. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab11936 at 1/250. The secondary antibody (shown in green) was ab180063 at 1/500 (Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) and counterstained using ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

  The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Syrian hamster monoclonal [RTD4E10] to Podoplanin - BSA and Azide free (ab11936)Image courtesy of an anonymous Abreview.

  ab11936 staining Podoplanin in murine skin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and an enzymatic antigen retrieval step was performed using Protein K. Samples were then blocked with 5% serum for 30 minutes at room temperature and then incubated with ab11936 at a 1/400 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated goat anti-hamster IgG used at a 1/500 dilution.

  See Abreview