Anti-Coilin antibody [Pdelta]

• 产品名称

  Anti-Coilin抗体[Pdelta]
  参阅全部 Coilin 一抗

• 描述

  小鼠单克隆抗体[Pdelta] to Coilin

• 宿主

  Mouse

• 经测试应用

  适用于: WB, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Recombinant fragment corresponding to Human Coilin (C terminal).

• 表位

  Monoclonal Anti-coilin reacts with an epitope located between amino acids 363 and 481 of human coilin.

• 阳性对照

• HeLa nuclear extract.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: 0.0268% PBS

• 浓度

• 50 µl 浓度为 1.9 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  Pdelta

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11822于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 8161 Human

• Entrez Gene: 12812 Mouse

• Omim: 600272 Human

• SwissProt: P38432 Human

• SwissProt: Q5SU73 Mouse

• Unigene: 532795 Human

• 别名

• COIL_HUMAN antibody

• Coilin antibody

• Coilin p80 antibody

• p80 antibody

• P80 coilin antibody

• CLN 80 antibody

• CLN80 antibody

• COIL antibody

Immunocytochemistry/ Immunofluorescence - Anti-Coilin antibody [Pdelta] (ab11822)

ICC/IF image of ab11822 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11822, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.