Anti-DCTN1/p150-glued抗体
参阅全部 DCTN1/p150-glued 一抗
山羊多克隆抗体to DCTN1/p150-glued
Goat
This antibody is expected to recognise both human isoforms.
适用于: IHC-P, WBmore details
与反应: Human
预测可用于: Rat, Drosophila melanogaster
Synthetic peptide corresponding to Human DCTN1/p150-glued aa 1266-1278 (C terminal).
Sequence:
C-QEQLHQLHSRLIS
(Peptide available as ab23214)
WB: HeLa cell lysates and MCF-7 cell lysates.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab11806于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) | Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB | (2) | Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 150 kDa. A 1 hour primary incubation is recommended for this product. Approx 150kDa band observed in A549 and Human Testis lysates |
Entrez Gene: 39536 Drosophila melanogaster
Entrez Gene: 1639 Human
Omim: 601143 Human
SwissProt: P13496 Drosophila melanogaster
SwissProt: Q14203 Human
Unigene: 12980 Drosophila melanogaster
Unigene: 516111 Human
Unigene: 11284 Rat
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p150(GLUED) DROSOPHILA HOMOLOG OF antibody
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Western blot - Anti-DCTN1/p150-glued antibody (ab11806)
Lane 1 : Anti-DCTN1/p150-glued antibody (ab11806) at 1 µg/ml
Lane 2 : Anti-DCTN1/p150-glued antibody (ab11806) at 0.5 µg/ml
Lane 1 : HeLa cell lysates
Lane 2 : MCF-7 cell lysates
Lysates/proteins at 35 µg per lane.
Predicted band size: 150 kDa
Detected by chemiluminescence.
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