Anti-Ganglioside GD3 antibody [R24]

• 产品名称

  Anti-Ganglioside GD3抗体[R24]

• 描述

  小鼠单克隆抗体[R24] to Ganglioside GD3

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, ICC/IF, IHC-Frmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human Ganglioside GD3. This antibody was raised against SK-MEL-28 melanoma cell line.

• 阳性对照

• ICC/IF: SH-SY5Y cells. IHC-P: Human melanoma tissue. IHC-Fr: Human melanoma tisue.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituents: 98.9% PBS, 1% BSA

• 浓度

• 500 µl 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Affinity purified from tissue culture supernatant.

• 克隆

  单克隆

• 克隆编号

  R24

• 同种型

  IgG3

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab11779于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 别名

• GD3 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Ganglioside GD3 antibody [R24] (ab11779)

  ICC/IF image of ab11779 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11779, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

• 

  Immunohistochemistry (Frozen sections) - Anti-Ganglioside GD3 antibody [R24] (ab11779)

  Frozen section of human melanoma tissue stained for Ganglioside GD3 with ab11779 at 1/40 dilution in immunohistochemical analysis.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ganglioside GD3 antibody [R24] (ab11779)

  IHC image of ab11779 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11779, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.