Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker

• 产品名称

  Anti-E Cadherin抗体[DECMA-1] - Intercellular Junction Marker
  参阅全部 E Cadherin 一抗

• 描述

  大鼠单克隆抗体[DECMA-1] to E Cadherin - Intercellular Junction Marker

• 宿主

  Rat

• 特异性

  Some customers have used this antibody successfully in human; however, our latest tests were unsuccessful and therefore we can no longer guarantee the reactivity of this species.

• 经测试应用

  适用于: ICC/IFmore details

• 种属反应性

  与反应: Mouse
  预测可用于: Cow, Dog

• 免疫原

  Full length protein. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• ICC/IF: M158 cells.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  DECMA-1

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11512于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 282637 Cow

• Entrez Gene: 12550 Mouse

• SwissProt: Q6R8F2 Cow

• SwissProt: P09803 Mouse

• Unigene: 35605 Mouse

• 别名

• cadherin 1 type 1 E-cadherin antibody

• Cadherin-1 antibody

• Cadherin1 antibody

• CAM 120/80 antibody

• CD 324 antibody

• CD324 antibody

• CD324 antigen antibody

• cdh1 antibody

• CDHE antibody

• E-Cad/CTF3 antibody

• E-cadherin antibody

• ECAD antibody

• Epithelial cadherin antibody

• epithelial calcium dependant adhesion protein antibody

• LCAM antibody

• Liver cell adhesion molecule antibody

• UVO antibody

• Uvomorulin antibody

• Arc 1 antibody

• CADH1_HUMAN antibody

• Cadherin 1 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512)

  ab11512 staining E Cadherin - Intercellular Junction Marker in M158 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11512 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  
  

  Also suitable in cells fixed with 100% methanol (5 min).