Anti-Proteasome 20S alpha 5/PSMA5抗体
参阅全部 Proteasome 20S alpha 5/PSMA5 一抗
兔多克隆抗体to Proteasome 20S alpha 5/PSMA5
Rabbit
适用于: IHC-P, ICC/IF, WBmore details
与反应: Human
预测可用于: Cow, Zebrafish
Synthetic peptide corresponding to Human Proteasome 20S alpha 5/PSMA5 aa 120-133.
Sequence:
ALQFGEEDADPGAM
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab11437于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 26.5 kDa (predicted molecular weight: 26.5 kDa). |
Entrez Gene: 5686 Human
Entrez Gene: 403011 Zebrafish
Omim: 176844 Human
SwissProt: P28066 Human
Unigene: 485246 Human
Proteasome alpha5 subunit antibody
Proteasome component 5 antibody
Proteasome subunit alpha type 5 antibody
Proteasome subunit alpha type-5 antibody
Proteasome subunit zeta antibody
Proteasome zeta chain antibody
PSA5_HUMAN antibody
PSC5 antibody
PSMA5 antibody
ZETA antibody
Macropain zeta chain antibody
Multicatalytic endopeptidase complex zeta chain antibody
Proteasome (prosome macropain) subunit alpha type 5 antibody
Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)
All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)
Lane 1 : HeLa cell extract.
Lane 2 : HeLa cell extract with blocking peptide.
Predicted band size: 26.5 kDa
Observed band size: 26.5 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)
Immunofluorescence of HeLa cells using ab11437.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)
IHC image of ab11437 staining in human liver carcinoma liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11437, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
