Anti-TRPM2抗体
参阅全部 TRPM2 一抗
兔多克隆抗体to TRPM2
Rabbit
适用于: IHC-P, WB, IPmore details
与反应: Rat, Human
预测可用于: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to Human TRPM2 aa 650-750.
Database link: 7226
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab11168于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. | |
| WB | 1/300. Predicted molecular weight: 171 kDa. | |
| IP | Use at 1-4 µg/mg of lysate. |
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Unigene: 369759 Human
Unigene: 276762 Mouse
Entrez Gene: 7226 Human
Entrez Gene: 28240 Mouse
Omim: 603749 Human
SwissProt: O94759 Human
SwissProt: Q5KTC0 Mouse
SwissProt: Q91YD4 Mouse
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Western blot - Anti-TRPM2 antibody (ab11168)
All lanes : Anti-TRPM2 antibody (ab11168) at 0.1 µg
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) membrane preparation at 50 µg
Lane 2 : Jurkat membrane preparation at 15 µg
Lane 3 : Jurkat membrane preparation at 5 µg
Developed using the ECL technique.
Predicted band size: 171 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM2 antibody (ab11168)
IHC image of ab11168 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11168, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunoprecipitation - Anti-TRPM2 antibody (ab11168)
Detection of human TRPM2 by western blot of immunoprecipitates. Samples: Membrane Prep (0.5 or 1.0mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPM2 antibody A300-414A (lot A300-414A-3) used for IP at 6ug per reaction. TRPM2 was also immunoprecipitated by rabbit anti-TRPM2 antibody A300-413A. For blotting immunoprecipitated TRPM2, A300-414A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
