Anti-beta Amyloid antibody [DE2B4]

• 产品名称

  Anti-beta Amyloid抗体[DE2B4]
  参阅全部 beta Amyloid 一抗

• 描述

  小鼠单克隆抗体[DE2B4] to beta Amyloid

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, IP, IHC-Fr, IHC-P, WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide, corresponding to amino acids 1-17 of Human beta Amyloid.

• 表位

  ab11132 recognizes an epitope in the region 8-16 of human beta Amyloid.

• 阳性对照

• IHC-P: Alzheimer's disease human brain; Mouse brain tissue. IHC-Fr: Mouse brain cortex. WB: CHO-K1 cell lysate. ICC/IF: HEK cells.

• 常规说明

  This product should be stored undiluted. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 纯度

  Protein A purified

• 纯化说明

  Purified from tissue culture supernatant.

• 克隆

  单克隆

• 克隆编号

  DE2B4

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11132于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 351 Human

• Omim: 104760 Human

• SwissProt: P05067 Human

• Unigene: 434980 Human

• 别名

• ABPP antibody

• AD1 antibody

• AICD-50 antibody

• AICD-57 antibody

• AICD-59 antibody

• AID(50) antibody

• AID(57) antibody

• AID(59) antibody

• Alzheimer disease amyloid protein antibody

• amyloid beta A4 protein antibody

• Amyloid intracellular domain 50 antibody

• Amyloid intracellular domain 57 antibody

• Amyloid intracellular domain 59 antibody

• amyloid of aging and alzheimer disease antibody

• APP antibody

• APPI antibody

• beta-amyloid peptide antibody

• Beta-APP40 antibody

• Beta-APP42 antibody

• C31 antibody

• Cerebral vascular amyloid peptide antibody

• CTFgamma antibody

• CVAP antibody

• Gamma-CTF(50) antibody

• Gamma-CTF(57) antibody

• Gamma-CTF(59) antibody

• peptidase nexin-II antibody

• PN-II antibody

• PreA4 antibody

• Protease nexin-II antibody

• S-APP-alpha antibody

• S-APP-beta antibody

• A4_HUMAN antibody

• AAA antibody

• ABETA antibody

图片

• 

  Immunohistochemistry (Frozen sections) - Anti-beta Amyloid antibody [DE2B4] (ab11132)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

  ab11132 staining beta Amyloid in transgenic APPP23 mouse brain cortex by immunohistochemistry (frozen sections). Unfixed cryosections were fixed in 4% Formalin in PBS before treatment with 70% Formic acid to reveal antigenic sites. Fixed samples were permeabilized with 0.1% Tween 20, blocked with 1% BSA for 30 minutes at 1oC. Samples were incubated with primary antibody (1/200 in TBS/BSA/Tween 20/azide) for 2 hours. Anti-mouse IgG Alexa Fluor® 594 (1/1000) was used as the secondary antibody.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-beta Amyloid antibody [DE2B4] (ab11132)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France

  Immunofluorescence detection using Mouse monoclonal [DE2B4] to beta Amyloid (ab11132) on Human HEK cells. Cells were fixed using Methanol, no antigen retrieval step was necessary. Primary antibody ab11132 was incubated for 1hr at room temperature (1/400). The confocal image of transfected HEK cells shows A. anti-amyloid beta (ab11132), B. swAPP-GFP, C. Overlay. Significant colocalisation is seen as would be expected. (GFP is tagged to C-term of swAPP).