Anti-PIP2 antibody [2C11]

• 产品名称

  Anti-PIP2抗体[2C11]

• 描述

  小鼠单克隆抗体[2C11] to PIP2

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, ICC/IF, ELISA, Neutralisingmore details

• 种属反应性

  与反应: Species independent
  

• 免疫原

  Chemical/ Small Molecule corresponding to PIP2. Liposomes containing synthetic dipalmitoyl PtdIns(4,5)P2 or PtdIns(3,4,5)P3

• 阳性对照

• IHC-P: FFPE human kidney normal and FFPE mouse normal brain. ICC/IF: HepG2 cell line, Neuro2a cell line

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
  
  Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  2C11

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgM

The Abpromise guarantee

Abpromise™承诺保证使用ab11039于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

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  Immunocytochemistry - Anti-PIP2 antibody [2C11] (ab11039)This image is courtesy of an anonymous Abreview

  Immunocytochemistry analysis of methanol-fixed 0.3% tritonX-100 permeabilized Bone Osteosarcoma Epithelial Cells staining with ab11039 at 1/200. Secondary antibody was Cy3® anti-mouse at 1/300 dilution. Samples were incubated with the primary antibody for 16 hours at 4°C. Blocking was done using 5% serum for 1 hour at 25°C.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-PIP2 antibody [2C11] (ab11039)

  ICC/IF image of ab11039 stained human HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11039, 5 µg/ml) overnight at +4°C.

  The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgM (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and MCF7 cells.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIP2 antibody [2C11] (ab11039)

  IHC image of PIP2 staining in mouse normal brain formalin fixed paraffin embedded tissue section. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11039, 1µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab98679, 1/1000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

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  Immunocytochemistry/ Immunofluorescence - Anti-PIP2 antibody [2C11] (ab11039)Image from Ozkucur N et al., BMC Cell Biol. 2011 Jan 22;12:4. Fig S4.; doi:10.1186/1471-2121-12-4; 22 January, 2011, BMC Cell Biology 2011, 12:4

  Immunofluorescence analysis of Human SaOS-2 (Human osteosarcoma) cells, staining PIP2 with ab11039. Cells were either unstimulated (upper panel) or stimulated with direct current (lower panel).
  
  Cells were fixed in formaldehyde, permeabilized and then blocked with 1% BSA for 20 min. Cells were then incubated with a primary antibody (1/200) overnight at 4°C. A FITC-conjugated anti-mouse IgG was used as the secondary antibody.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIP2 antibody [2C11] (ab11039)

  IHC image of PIP2 staining in a formalin fixed, paraffin embedded human normal kidney tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11039, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

   

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre