Anti-Glucagon antibody [K79bB10]

• 产品名称

  Anti-Glucagon抗体[K79bB10]
  参阅全部 Glucagon 一抗

• 描述

  小鼠单克隆抗体[K79bB10] to Glucagon

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-Pmore details

• 种属反应性

  与反应: Rat
  预测可用于: Rabbit, Guinea pig, Cat, Dog, Human, Pig, Common marmoset, Syrian hamster

• 免疫原

  Full length protein corresponding to Pig Glucagon. Polymerized porcine glucagon.
  Database link: P01274

• 阳性对照

• Pancreatic glucagon for RIA. IHC-P: Mouse and Rat pancreas tissues.

• 常规说明

  Production of this antibody has been changed on 8th July 2016. The following lots are from ascites and are still in stock as of 8th July 2016: GR260160 and GR268340.  Lot numbers higher than GR268340 will be purified from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

  If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 批次浓度范围 50 µl 浓度为 1.2 - 1.3 mg/ml

• 纯度

  Proprietary Purification

• 纯化说明

  Purified from Tissue culture supernatant.

• 克隆

  单克隆

• 克隆编号

  K79bB10

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab10988于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• SwissProt: P06883 Rat

• Entrez Gene: 403571 Dog

• Entrez Gene: 2641 Human

• Entrez Gene: 397595 Pig

• Entrez Gene: 24952 Rat

• Omim: 138030 Human

• SwissProt: P01275 Human

• SwissProt: P01274 Pig

• SwissProt: P68274 Rabbit

• 别名

• GCG antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)

  Formali-fixed, paraffin-embedded rat pancreas tissue stained for Glucagon using ab10988 at 10 µg/ml in immunohistochemical analysis.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)Image courtesy of Thomas Schoeppe by Abreview.

  ab10988 staining Glucagon in rat pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  Tissue was fixed in paraformaldehyde, blocked with 2% serum for 1 hour at 25°C, then incubated with ab10988 at a 1/200 dilution for 1 hour at 25°C. The secondary used was a donkey anti-mouse IgG (H+L) conjugated to Texas Red, used at a 1/250 dilution. Nuclei counterstained with DAPI, green is staining for insulin.

  See Abreview